Delivery of nucleic acid-like compounds
View Patent ↗Provided herein is a process for preparing a microparticulate complex of a particle-forming component (“PFC”) and a nucleic acid-like component (“NAC”) in a monophasic composition comprising water and a water-miscible organic solvent. Also provided is a microparticulate complex that comprises a particle-forming component complexed to a nucleic acid-like component, and a composition comprising water and the microparticulate complex. Further provided is a method of delivery of a nucleic acid-like component to a cell or to a patient by contacting the cell with or administering to the patient a composition comprising water and the microparticulate complex described herein. Still further, a charge-changing composition represented by the formula A-X—B is provided.
1. A process for preparing a microparticulate complex of lipid an nucleic acid, which process comprises:
combining
a lipid composition comprising cationic and non-cationic lipids, and
a nucleic acid component,
in an aqueous/organic solvent system comprising water and less than 55% by volume of a water-miscible organic solvent, wherein the aqueous component of the aqueous/organic solvent system has an ionic strength of less than that of about 50 mM NaCl,
to form a mixture which is heated to a temperature of from 30° to 80° C.,
forming a monophasic system wherein the aqueous/organic solvent system is a single liquid phase and wherein the lipid composition and the nucleic acid component are independently molecularly or micellarly soluble in the single liquid phase, and
reducing the amount of the organic solvent in the monophasic system so that the microparticulate complex is formed.
2. The process of claim 1 , wherein, prior to combining the lipid composition and the nucleic acid component, the cationic lipid is dissolved in a mixture of water and organic solvent and the nucleic acid component is in an aqueous composition.
3. The process of claim 1 , wherein the microparticulate complex is in the form of particles that are approximately spherical and range in size from about 60 nm to about 350 nm.
4. The process of claim 1 , wherein subsequent to the formation of the microparticulate complex, the microparticulate complex is separated from the liquid phase.
5. The process of claim 4 wherein the separation is carried out by size exclusion chromatography.
6. The process of claim 1 , in which a ligand is incorporated into the microparticulate complex.
7. The process of claim 6 , wherein the ligand is conjugated to a lipophilic anchor through a hydrophilic polymer linker.
8. The process of claim 1 , wherein an amphiphilic lipid is mixed with the mixture when the lipid composition and the nucleic acid component are combined or when the amount of the organic solvent in the monophasic system is reduced.
9. The process of claim 8 , wherein the amphiphilic lipid is a hydrophilic polymer-lipid conjugate, polyoxethylene ether of a fatty alcohol, a polyoxyethylene ester of a fatty acid, or a polyethylene ether of a polyol derivatized with a fatty alcohol or a fatty acid.
10. The process of claim 9 , wherein the hydrophilic polymer is a poly(ethylene glycol) having a molecular weight of about 250 to about 20,000 and the lipid is a phospholipid, a sphingolipid, a diacylglyerol, or a sterol.
11. The process of claim 8 , wherein the amphiphilic lipid is present in an amount less than about 5.0 mol. %.
12. The process of claim 1 , wherein the water-miscible organic solvent is a water-miscible alcohol or ether.
13. A process for preparing a microparticulate complex of lipid and nucleic acid, which process comprises:
combining
a lipid composition comprising cationic and non-cationic lipids, and
a nucleic acid component,
in an aqueous/organic solvent system consisting essentially of water and less than 55% by volume of a water-miscible organic solvent, wherein the aqueous component of the aqueous/organic solvent system has an ionic strength of less than that of about 50 mM NaCl,
to form a mixture which is heated to a temperature of from 30° to 80° C.,
forming a monophasic system wherein the aqueous/organic solvent system is substantially a single liquid phase and wherein the lipid composition and the nucleic acid component are independently molecularly or micellarly soluble in the single liquid phase, and
reducing the amount of the organic solvent in the monophasic system so that the microparticulate complex is formed.
14. A process for preparing a microparticulate complex of lipid and nucleic acid, which process comprises:
combining
a lipid composition comprising cationic and non-cationic lipids, and
a nucleic acid component,
in an aqueous/organic solvent system comprising water and, in an amount from 40% to 55% by volume, a water-miscible alcohol or ether that is essentially free of halogenated hydrocarbon, wherein the aqueous component of the aqueous/organic solvent system has an ionic strength of less than that of about 50 mM NaCl,
to form a mixture which is heated to a temperature of from 30° to 80° C.,
forming a monophasic system wherein the aqueous/organic solvent system consists essentially of a single liquid phase and wherein the lipid composition and the nucleic acid component are independently molecularly or micellarly soluble in the single liquid phase, and
reducing the amount of the alcohol or ether in the monophasic system so that the microparticulate complex is formed.
15. The process of claim 14 , wherein, prior to combining the lipid composition and the nucleic acid component, the cationic lipid is dissolved in a mixture of water and alcohol or ether and the nucleic acid component is in an aqueous composition.
16. The process of claim 14 , wherein the microparticulate complex is in the form of particles that are approximately spherical and range in size from about 60 nm to about 350 nm.
17. The process of claim 14 , wherein subsequent to the formation of the microparticulate complex, the microparticulate complex is separated from the liquid phase.
18. The process of claim 17 wherein the separation is carried out by size exclusion chromatography.
19. The process of claim 14 , in which a ligand is incorporated into the microparticulate complex.
20. The process of claim 19 , wherein the ligand is conjugated to a lipophilic anchor through a hydrophilic polymer linker.
21. The process of claim 14 , wherein an amphiphilic lipid is mixed with the mixture when the lipid composition and the nucleic acid component are combined or when the amount of the alcohol or ether in the monophasic system is reduced.
22. The process of claim 21 , wherein the amphiphilic lipid is a hydrophilic polymer-lipid conjugate, a polyoxethylene ether of a fatty alcohol, a polyoxyethylene ester of a fatty acid, or a polyethylene ether of a polyol derivatized with a fatty alcohol or a fatty acid.
23. The process of claim 22 , wherein the hydrophilic polymer is a poly(ethylene glycol) having a molecular weight of about 250 to about 20,000 and the lipid is a phospholipid, a sphingolipid, a diacylglyerol, or a sterol.
24. The process of claim 21 , wherein the amphiphilic lipid is present in an amount less than about 5.0 mol. %.