Molecular markers for various traits in wheat and methods of use
This disclosure relates to methods of identifying and/or selecting wheat plants or germplasm by detecting markers associated with flowering date, anther-extrusion, heading date and/or fusarium head blight resistance.
1. A method of producing a wheat plant or germplasm that displays improved fusarium head blight resistance, said method comprising:
(a) isolating a nucleic acid from a wheat plant or part thereof;
(b) detecting a C allele at nucleotide position 101 of SEQ ID NO:149,
(c) thereby identifying and/or selecting a fusarium head blight resistant wheat plant;
(d) crossing the wheat plant of (c) with a second wheat plant not having the C allele at nucleotide position 101 of SEQ ID N 0 :149;
(e) collecting seed from the cross in step (d); and
(f) growing a progeny wheat plant from said seed which comprises the C allele at nucleotide position 101 of SEQ ID N 0 :149, thereby producing a wheat plant with improved fusarium head blight resistance relative to a wheat plant not comprising the C allele at nucleotide position 101 of SEQ ID N 0 :149.
2. A method of producing a wheat plant or germplasm with improved fusarium head blight resistance, said method comprising:
(a) isolating a nucleic acid from a wheat plant or part thereof;
(b) detecting a haplotype associated with the improved fusarium head blight resistance, wherein said haplotype comprises: a G allele at nucleotide position 101 of SEQ ID NO:259, an A allele at nucleotide position 101 of SEQ ID NO:263, a T allele at nucleotide position 46 of SEQ ID NO:240, a C allele at nucleotide position 101 of SEQ ID NO:149, a C allele at nucleotide position 101 of SEQ ID NO:264, a G allele at nucleotide position 101 of SEQ ID NO:176, and a T allele at nucleotide position 101 of SEQ ID NO:272;
(c) thereby identifying and/or selecting a fusarium head blight resistant wheat plant;
(d) crossing the wheat plant of (c) with a second wheat plant not having the haplotype;
(e) collecting seed from the cross in step (d); and
(f) growing a progeny wheat plant from said seed which comprises said haplotype in its genome, thereby producing a wheat plant with improved fusarium head blight resistance relative to a wheat plant not comprising the haplotype.