Microorganisms expressing ketoacyl-CoA synthase and uses thereof
View Patent ↗Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.
1. A recombinant oleaginous cell of the genus Prototheca , the cell comprising an exogenous gene encoding an active ketoacyl-CoA synthase enzyme of Cramble abyssinica, Lunaria annua or Cardamine graeca , wherein the cell produces an oil elevated in erucic acid due to the expression of the exogenous gene.
2. The cell of claim 1 , wherein the cell produces an oil comprising greater than 5% erucic acid.
3. The cell of claim 2 , wherein the cell produces an oil comprising greater than 10% erucic acid.
4. The cell of claim 2 , wherein the cell comprises at least 40% oil.
5. The cell of claim 1 , wherein the cell is of the species Prototheca moriformis.
6. A method for producing an oil comprising providing or cultivating a cell according to claim 1 and extracting an oil from the cell.
7. A recombinant oleaginous cell of the genus Prototheca , the cell comprising an exogenous gene encoding an active ketoacyl-CoA synthase enzyme of Cramble abyssinica, Lunaria annua or Cardamine graeca , wherein the cell produces an oil elevated in eicosenoic acid due to the expression of the exogenous gene.
8. The cell of claim 7 , wherein the cell produces an oil comprising greater than 5% eicosenoic acid.
9. The cell of claim 7 , wherein the cell produces an oil comprising greater than 10% eicosenoic acid.
10. The cell of claim 7 , wherein the cell comprises at least 40% oil.
11. A recombinant oleaginous cell of the genus Prototheca , the cell comprising an exogenous gene encoding an active ketoacyl-CoA synthase enzyme of Cramble abyssinica, Lunaria annua or Cardamine graeca , wherein the cell produces an oil elevated in docosadienoic acid due to the expression of the exogenous gene.
12. A recombinant oleaginous cell of the genus Prototheca , the cell comprising an exogenous gene encoding an active ketoacyl-CoA synthase enzyme of Cramble abyssinica, Lunaria annua or Cardamine graeca , wherein the cell produces an oil elevated in C22:5 due to the expression of the exogenous gene.