Generation of plants with altered protein, fiber, or oil content
The present disclosure is directed to plants that display an improved oil quantity phenotype or an improved meal quality phenotype due to altered expression of an IMQ nucleic acid. The disclosure is further directed to methods of generating plants with an improved oil quantity phenotype or improved meal quality phenotype.
1. A method of producing an improved meal quality phenotype in a plant, said method comprising:
a) introducing into progenitor cells of the plant a plant transformation vector comprising a nucleotide sequence that encodes an IMQ polypeptide comprising an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO: 72;
b) growing the transformed progenitor cells to produce a transgenic plant, wherein the nucleotide sequence is expressed, and the transgenic plant exhibits an improved meal quality phenotype, wherein the improved meal quality phenotype comprises increased digestible protein and/or decreased fiber relative to control plants; and
c) measuring the improved meal quality phenotype in the transgenic plant relative to the control plants,
thereby producing the improved meal quality phenotype in the plant.
2. A plant obtained by the method of claim 1 .
3. The plant of claim 2 , which is selected from the group consisting of plants of the Brassica species, including canola and rapeseed, soy, corn, sunflower, cotton, cocoa, safflower, oil palm, coconut palm, flax, castor, peanut, wheat, oat, and rice.
4. The plant of claim 2 , wherein the plant is selected from the group consisting of a plant grown from said progenitor cells, a plant that is the direct progeny of a plant grown from said progenitor cells, and a plant that is the indirect progeny of a plant grown from said progenitor cells.
5. A method of generating a plant having an improved meal quality phenotype comprising:
identifying a plant that has an allele in its ortholog of the A. thaliana IMQ gene which results in improved meal quality phenotype compared to plants lacking the allele, by employing candidate gene/QTL methodology or TILLING methodology, where the wildtype A. thaliana gene has the nucleic acid sequence set forth as SEQ ID NO: 71 and wherein the ortholog comprises a nucleic acid sequence at least 80% identical to SEQ ID NO: 71; and
generating progeny of said identified plant, wherein the generated progeny inherit the allele and have the improved meal quality phenotype, thereby generating a plant having an improved meal quality phenotype.
6. The method of claim 1 , wherein the IMQ polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 72.
7. The method of claim 1 , wherein the IMQ polypeptide consists of the amino acid sequence set forth in SEQ ID NO: 72.
8. The method of claim 1 , wherein the nucleotide sequence is operably linked to a constitutive, inducible, or regulatable promoter sequence.