Enzymes for improved biomass conversion
Disclosed herein are enzymes and combinations of the enzymes useful for the hydrolysis of cellulose and the conversion of biomass. Methods of degrading cellulose and biomass using enzymes and cocktails of enzymes are also disclosed.
1. A method for degrading cellulose, comprising contacting a cellulose containing material with an enzyme cocktail comprising a recombinant thermostable enzyme from Caldicellulosiruptor bescii comprising a glycoside hydrolase (GH9) domain and a glycoside hydrolase (GH48) domain and a recombinant thermostable β-glucosidase, wherein at least 50% of the cellulose in the material is converted to at least one sugar.
2. The method of claim 1 , wherein the thermostable enzyme comprising a GH9 domain and a GH48 domain is Caldicellulosiruptor bescii CelA.
3. The method of claim 1 , wherein the thermostable β-glucosidase is from a bacterium of the genus Thermotoga.
4. The method of claim 1 , wherein the enzyme cocktail further comprises a thermostable endoglucanase.
5. The method of claim 4 , wherein the thermostable endoglucanase is from a bacterium of the genus Acidothermus.
6. The method of claim 4 , wherein the thermostable endoglucanase is Acidothermus cellulolyticus E1.
7. A method for degrading lignocellulosic biomass, comprising contacting the lignocellulosic biomass with an enzyme cocktail comprising a recombinant thermostable enzyme from Caldicellulosiruptor bescii comprising a glycoside hydrolase (GH9) domain and a glycoside hydrolase (GH48) domain and a recombinant thermostable β glucosidase, wherein at least 50% of the cellulose in the biomass is converted to at least one sugar.
8. The method of claim 7 , wherein the thermostable enzyme comprising a GH9 domain and a GH48 domain is Caldicellulosiruptor bescii CelA.
9. The method of claim 7 , wherein the thermostable β-glucosidase is from a bacterium of the genus Thermotoga.
10. The method of claim 7 , wherein the enzyme cocktail further comprises a thermostable endoglucanase.
11. The method of claim 10 , wherein the thermostable endoglucanase is from a bacterium of the genus Acidothermus.
12. The method of claim 1 , wherein the thermostable enzyme comprising a GH9 domain and a GH48 domain comprises at least 30% of the enzyme cocktail.
13. The method of claim 1 , wherein the thermostable enzyme comprising a GH9 domain and a GH48 domain comprises at least 50% of the enzyme cocktail.
14. The method of claim 1 , wherein the thermostable enzyme comprising a GH9 domain and a GH48 domain is Caldicellulosiruptor bescii CelA, wherein the thermostable β-glucosidase is from the bacterium Thermotoga maritima ; and wherein the CelA comprises at least 30% of the enzyme cocktail and the β-glucosidase comprises at least about 5% of the enzyme cocktail.
15. The method of claim 14 , wherein the enzyme cocktail further comprises at least 5% of the thermostable endoglucanase Acidothermus cellulolyticus E1.
16. A method for degrading cellulose, comprising contacting a cellulose containing material with an enzyme cocktail comprising CelA from Caldicellulosiruptor bescii and a thermostable β-glucosidase from Thermotoga maritima;
wherein the thermostable β-glucosidase comprises about 5% of the enzyme cocktail by weight; and
wherein at least 50% of the cellulose in the material is converted to at least one sugar.
17. The method of claim 16 , wherein at least 70% of the cellulose in the material is converted to at least one sugar.
18. The method of claim 16 , wherein 50 to 100% of the cellulose in the material is converted to at least one sugar.
19. The method of claim 16 , wherein 70 to 100% of the cellulose in the material is converted to at least one sugar.