Scanner photometer and methods
A scanning photometer and attendant methods are provided. The scanning photometer is generally characterized by first and second fluorophore excitation sources, an objective lens, and a common emission detector for the detection of first and second fluorophore emission originating from the excitation of the fluorophores via passage of excitation energy, via an optical path of the objective lens, from the excitation sources. Excitation energy and emission energy conditioning elements are likewise provided, operatively interposed before or after the objective lens as the case may be.
1. A photometric scanning system comprising a photometer and a scanning rail carriage, said photometer operatively supported by said scanning rail carriage for reversible travel with respect to a scanning region within which an optical target is positionable, said photometer comprising:
(a). a first fluorophore excitation source;
(b). a second fluorophore excitation source;
(c). an objective lens, characterized by an optical path, through which excitation energy from said first and said second fluorophore excitation sources pass; and,
(d). a first emission detector for detecting both a first fluorophore emission originating from excitation of a first fluorophore and a second fluorophore emission originating from excitation of a second fluorophore.
2. The photometric scanning system of claim 1 further comprising a third fluorophore excitation source, excitation energy from said third fluorophore excitation source passing through said objective lens.
3. The photometric scanning system of claim 1 further comprising a third fluorophore excitation source and a second emission detector for detecting a third fluorophore emission originating from excitation of a third fluorophore.
4. The photometric scanning system of claim 1 further comprising excitation energy conditioning elements operatively interposed between said first and second excitation sources and said objective lens.
5. The photometric scanning system of claim 4 wherein said excitation energy conditioning elements comprise excitation filters and beam splitters for select passage of excitation energy to and along said optical path of said objective lens.
6. The photometric scanning system of claim 4 further comprising emission energy conditioning elements operatively interposed between said objective lens and said first emission detector.
7. The photometric scanning system of claim 6 wherein said emission energy conditioning elements comprise beam splitters and an emission filter for select passage of emission energy to said first emission detector.
8. The photometric scanning system of claim 1 further comprising a first beam splitter, excitation energy from one of said first fluorophore excitation source and said second fluorophore excitation source being reflected thereby.
9. The photometric scanning system of claim 8 further comprising a second beam splitter, excitation energy from both of said first fluorophore excitation source and said second fluorophore excitation source being reflected thereby.
10. The photometric scanning system of claim 1 further comprising emission energy conditioning elements operatively interposed between said objective lens and said first emission detector.