Methods for performing thermal melt analysis
View Patent ↗The present disclosure provides apparatus, systems, and methods for conducting rapid, accurate, and consistent heated amplifications and/or thermal melt analyses.
1. A method for performing a thermal melt analysis on the contents of a receptacle contained within a thermal melt analysis module, the method comprising the steps of:
(a) bringing a receptacle into thermal contact with a thermal block contained within the thermal melt analysis module, wherein the contents of the receptacle are at an initial temperature that is lower than the temperature of the thermal block when the receptacle is brought into thermal contact with the thermal block;
(b) allowing the receptacle to dwell in thermal contact with the thermal block for a predetermined dwell period so that the temperature of the contents of the receptacle increases from the initial temperature to a temperature that is higher than the initial temperature;
(c) during step (b), periodically measuring an optical signal emitted from the contents of the receptacle as the temperature of the contents of the receptacle increases;
(d) detecting a change, if any, in the measured optical signal during step (c); and
(e) removing the receptacle from the thermal melt analysis module,
wherein the thermal block is maintained at a steady-state temperature during steps (a)-(d).
2. The method of claim 1 , wherein steps (a)-(e) are repeated with two or more receptacles and the temperature of the thermal block is maintained at the steady-state temperature between repeating steps (a)-(e) with the two or more receptacles.
3. The method of claim 1 , wherein the steady-state temperature of the thermal block is at least 90° C.
4. The method of claim 1 , wherein the steady-state temperature of the thermal block is from 70° C. to 120° C.
5. The method of claim 1 , wherein the steady-state temperature of the thermal block is from 70° C. to 90° C.
6. The method of claim 1 , wherein the change in the measured optical signal, if any, is associated with the melting of the hydrogen bonds between hybridized nucleic acid sequences contained in the receptacle.
7. The method of claim 1 , wherein steps (a)-(e) are accomplished in less than about 5 minutes.
8. The method of claim 1 , wherein detecting a change in the measured optical signal comprises computing a derivative of the optical signal with respect to temperature and identifying an inflection point in the optical signal.
9. The method of claim 1 , wherein multiple different optical signals emitted from the contents of the receptacle are monitored.
10. The method of claim 1 , further comprising performing a nucleic acid amplification procedure with the contents of the receptacle prior to step (a).