Animal protein free media for cultivation of cells
View Patent ↗The present invention relates to animal protein free cell culture media comprising a combination of non-animal derived peptides derived from soy hydrolysate and yeast hydrolysate. The invention also provides an animal protein free culture process, wherein cells are cultivated, propagated and passaged without animal-derived components. This process is useful for cultivating cells, such as recombinant cells or cells infected with a virus, and for production biological products by cell culture processes under conditions devoid of animal protein components.
1. A method for producing a virus, comprising:
growing a culture of VERO cells in an animal protein free medium comprising soy hydrolysate at a concentration of about 0.05% (w/v) to about 1% (w/v) and yeast hydrolysate at a concentration of about 0.05% (w/v) to about 0.3% (w/v);
infecting the VERO cells with a virus; and
incubating the infected VERO cells to propagate the virus.
2. The method of claim 1 , wherein the virus is selected from the group consisting of vaccinia virus, coronavirus, orthomyxovirus, Ross River virus, Flavivirus, and picornavirus.
3. The method of claim 2 , wherein the virus is an orthomyxovirus selected from the group consisting of influenza A, influenza B, and influenza C virus.
4. The method of claim 2 , wherein the virus is a Flavivirus selected from the group consisting of Yellow fever virus, Japanese encephalitis virus, Tick-borne encephalitis virus, West nile Virus and Hepatitis C virus.
5. The method of claim 2 , wherein the virus is a picornavirus selected from the group consisting of poliovirus and hepatitis A virus.