Cartilage material
View Patent ↗Cartilage materials such as cartilage fluff and a cartilage composition comprising a particulate material are disclosed. These are suitable for stimulating chondrogenesis and/or producing cartilage regeneration. Also disclosed are processes for their preparation. Methods for regenerating articular cartilage are also disclosed, which involve, for example, placing the cartilage fluff or cartilage composition into a cartilage defect.
1. A method of preparing a cartilage composition comprises the steps of:
a) obtaining a hyaline cartilage material, the material being in the form of pieces of cartilage non-demineralized and not subjected to demineralization treatments or harsh chemicals which adversely affect the material properties of the cartilage material and not subjecting the material to elevated temperatures greater than 50 degrees C.;
b) washing the cartilage material in normal saline or balanced salt solution using several washes;
c) rapidly freezing and drying the material to about 3 to 5 percent moisture content;
d) preserving the pieces of cartilage material by freeze drying, freezing or cryo-preserving and/or dried;
e) intermittently grinding, milling or shaving the preserved pieces of cartilage material without raising the material temperature above 40 degrees C. to form a ground, milled or shaved material of a cartilage fluff and cartilage particles;
f) sieving the cartilage fluff and particles through a sieve having openings up to 1000 μm after each intermittent grinding;
g) allowing the cartilage particles less than the size of the sieve openings to pass;
h) collecting the cartilage fluff made of a plurality of branched strands intertwined to form a self-supporting matrix of cartilage fluff, each strand being 1000 μm to 6000 μm, to be formed in the sieve, the cartilage fluff having a cohesive characteristic wherein the cartilage fluff sticks to itself to form a continuous network of intertwined branched strands of cartilage fluff packing material having a physical configuration resembling a cotton composition for packing into defects in articular cartilage or bone; and
i) followed by infusing the cartilage fluff with blood.
2. The method of claim 1 further comprises the step of: securing bone with cartilage in a milling and digitizing machine, and shaving the cartilage to obtain the cartilage fluff.
3. The method of claim 2 , wherein the shaving is carried out with a drill bit, core drill, or burr.
4. The method of claim 1 further comprises the step of: freeze-drying cartilage and milling the freeze-dried cartilage to obtain the cartilage fluff.
5. The method of claim 4 , wherein said cartilage is attached to bone.
6. The method of claim 4 , wherein said cartilage is unattached to bone.
7. The method of claim 1 wherein the step of: (d) preserving the pieces of cartilage material is by freezing or by cryo-preserving the cartilage.
8. The method of claim 1 further comprises the step of: adding one or more additional cartilage growth promoting factors to the cartilage fluff.
9. The method of claim 8 further comprises the step of:
adding one or more of chondrogenic stimulating factors to the cartilage fluff including, growth factors FGF-2, FGF-5, IGF-1, TGF-.beta., BMP-2, BMP-7, PDGF, VEGF, human allogenic or autologous chondrocytes, human allogenic or autologous bone marrow cells, stem cells, demineralized bone matrix, insulin, insulin-like growth factor-1, transforming growth factor-B, interleukin-1 receptor antagonist, hepatocyte growth factor, platelet-derived growth factor, Indian hedgehog and parathyroid hormone-related peptide or bioactive glue.
10. The method of claim 1 , wherein the step of sieving includes using a sieve having 850 μm to 1000 μm openings.
11. The method of claim 10 , wherein the cartilage fluff is in the range of 1000 μm to 3000 μm.