Control of Cyanate in Aqueous Urea Solutions by Non-1,2-Ethylene Diamine Like Compounds for the Protection of Protein/Peptide Carbamylation
Embodiments of the present invention generally relate to processing of peptides in urea solutions and substantial prevention of carbamylation of the peptide.
1 - 19 . (canceled)
20 . A method of inhibiting and/or delaying carbamylation of a polypeptide in a urea and/or cyanate containing solution, the method comprising a step of adding a carbamylation-inhibiting compound to the solution,
wherein said carbamylation-inhibiting compound is selected from the group consisting of L-Glycine, L-histidine, Diethanolamine, L-Arginine, L-Threonine, L-Lysine, L-Cysteine, Taurine, Hydralazine, 4-Hydroxy-Proline, a dipeptide and Tri-Glycine.
21 . The method according to claim 20 , wherein the polypeptide is a ribonuclease.
22 . The method according to claim 21 , wherein the ribonuclease is RNase A.
23 . The method according to claim 20 , wherein the carbamylation-inhibiting compound is added to the solution in an amount effective to provide about 100% carbamylation protection of the polypeptide for a period of three weeks.
24 . The method according to claim 20 , wherein the concentration of the carbamylation-inhibiting compound is between 1 mM and 150 mM.
25 . The method according to claim 20 , wherein the carbamylation-inhibiting compound has a buffering capacity of about neutral.
26 . A method of inhibiting and/or delaying carbamylation of a polypeptide in a urea and/or cyanate containing solution, the method comprising a step of adding a carbamylation-inhibiting compound to the solution,
wherein the carbamylation-inhibiting compound is selected from the group consisting of L-Glycine, L-histidine, Diethanolamine, L-Arginine, L-Threonine, L-Lysine, L-Cysteine, Taurine, Hydralazine, 4-Hydroxy-Proline, Glycinamide, a dipeptide and Tri-Glycine
27 . The method according to claim 26 , wherein the carbamylation-inhibiting compound has a buffering capacity of about neutral.
28 . A method of inhibiting and/or delaying carbamylation of a polypeptide in a urea and/or cyanate containing solution, the method comprising a step of adding a carbamylation-inhibiting compound selected from the group consisting of L-Glycine, Diethanolamine, L-Arginine, L-Threonine, L-Lysine, L-Cysteine, Taurine, Hydralazine, Glycinamide, a dipeptide and Tri-Glycine to the solution,
wherein the carbamylation-inhibiting compound is added to the solution in an amount effective to provide about 100% carbamylation protection of the polypeptide for a period of three weeks.
29 . The method according to claim 28 , wherein the concentration of the carbamylation-inhibiting compound is between 1 mM and 150 mM.
30 . The method according to claim 29 , wherein the cyanate in the solution is at a concentration of about 5 mM.