Antibodies to aripiprazole haptens and use thereof
Disclosed is an antibody which binds to aripiprazole, which can be used to detect aripiprazole in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of aripiprazole, including multiplex detection of aripiprazole, olanzapine, quetiapine, and risperidone in a single lateral flow assay device.
1. An isolated antibody or a binding fragment thereof, which specifically binds to both aripiprazole and dehydroaripiprazole and which is generated in response to a conjugate of a compound of Formula I and an immunogenic carrier,
wherein:
R 1 is H,
CH 2 NH 2 or CH 2 NHC(O)(CH 2 ) m CO 2 H;
R 2 is H,
NH 2 , or NHC(O)(CH 2 ) m CO 2 H;
R 3 is H; provided that either R 1 or R 2 must be H, and further provided that R 1 and R 2 must not be H simultaneously;
m is 1, 2, 3, 4, or 5; and
n is 1, 2, 3, 4, or 5; wherein the isolated antibody does not bind to olanzapine, quetiapine and risperidone.
2. The antibody of claim 1 , wherein the immunogenic carrier is a protein.
3. The antibody of claim 1 , wherein the antibody fragment is selected from the group of fragments consisting of Fv, F(ab′), F(ab′)2, scFv, minibody and diabody fragments.
4. The antibody of claim 1 , wherein the antibody is a monoclonal antibody.
5. An assay kit comprising the antibody of claim 1 .
6. An assay device comprising the antibody of claim 1 .
7. The assay device of claim 6 wherein the device is a lateral flow assay device.