Cross-linking polypeptide that induces apoptosis
The disclosure relates to a polypeptide comprising at least four domains specifically binding to a certain MHC peptide complex, the domains separated by linker amino acid sequences, thereby providing each domain with the capability to bind a separate MHC peptide complex, to a nucleic acid encoding for such a polypeptide, to a vector comprising such a nucleic acid, to a host cell for expression of such a polypeptide, to a pharmaceutical composition comprising such a polypeptide, and to a kit of parts comprising at least two polypeptides according to the disclosure.
1. A polypeptide comprising Hexa-AH5: (SEQ ID NO:4), or Hexa-11HCH1: (SEQ ID NO:13), or Hexa-11HAH5: (SEQ ID NO:17).
2. The polypeptide of claim 1 , wherein the linker amino acid sequences allow for protein folding.
3. The polypeptide of claim 1 , which comprises SEQ ID NO:4.
4. The polypeptide of claim 1 , which comprises SEQ ID NO: 13.
5. The polypeptide of claim 1 , which comprises SEQ ID NO: 17.
6. A pharmaceutical composition comprising: the polypeptide of claim 1 , and a suitable diluent and/or excipient.
7. The pharmaceutical composition according to claim 6 , further comprising a cytostatic and/or tumoricidal agent.
8. A conjugate of a polypeptide of claim 1 , and a cytostatic or tumoricidal agent.
9. A method for producing the polypeptide of claim 1 , the method comprising: culturing a host cell comprising a polynucleotide encoding the polypeptide, allowing for expression of the polynucleotide, and harvesting the polypeptide.