Method for modifying nucleic acids
The present invention pertains to: a method for modifying a nucleic acid contained in a sample, the method including a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, the method including the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.
1. A method of modifying a nucleic acid contained in a sample solution, the method comprising:
a step of bringing the sample solution into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide,
a step of carrying out a light irradiation treatment, thereby modifying the nucleic acid in the sample solution;
wherein the acid polysaccharide is artificially added to the sample solution and then the sample solution is treated with the nucleic acid modifying agent,
wherein the nucleic acid-modifying agent is a compound capable of modifying a nucleic acid by photoactivation, and
wherein the acidic polysaccharide is selected from sodium alginate and chondroitin sulfate B.
2. The method according to claim 1 , wherein the nucleic acid-modifying agent is a compound selected from ethidium monoazide and propidium monoazide.
3. A method of selectively detecting a nucleic acid obtained from a living cell contained in a sample solution, the method comprising the following steps: (a) a step of bringing the sample solution into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide; (b) a step of carrying out a light irradiation treatment after step (a), thereby modifying a nucleic acid derived from a dead cell contained in the sample solution; and
(c) a step of selectively detecting an unmodified nucleic acid from the sample solution after the step (b), wherein the sample solution is a mixture of a living cell and a dead cell, wherein the acidic polysaccharide is selected from sodium alginate and chondroitin sulfate B.
4. The method according to claim 3 , wherein the nucleic acid-modifying agent is a compound selected from ethidium monoazide and propidium monoazide.
5. The method according to claim 3 , wherein the step (c) is performed by a nucleic acid amplification method.
6. The method according to claim 5 , wherein the step (c) is performed by real-time PCR.