Patent
The present invention relates to Brassica plants comprising mutant ALC genes, ALC nucleic acid sequences and proteins, as well as methods for generating and identifying said plants and alleles, which can be used to plants with increased podshatter resistance.
1. A Brassica napus plant, or a cell, part, seed or progeny thereof, comprising two non-naturally occurring full knock-out alc genes, wherein one non-naturally occurring full knock-out alc gene is a mutated version of the native ALC gene comprising at least 98% sequence identity to SEQ ID NO: 3 or encoding an amino acid sequence comprising at least 98% sequence identity to SEQ ID NO: 9; and wherein one non-naturally occurring full knock-out alc gene is a mutated version of the native ALC gene comprising at least 98% sequence identity to SEQ ID NO: 4 or encoding an amino acid sequence comprising at least 98% sequence identity to SEQ ID NO: 10;
wherein said full knock-out mutant alc gene comprises a mutated DNA region consisting of one or more inserted, deleted or substituted nucleotides compared to a corresponding wild-type DNA region in the functional ALC gene and wherein said mutant alc allele does not encode a functional ALC protein,
wherein the podshatter resistance is increased as compared to the podshatter resistance of a corresponding plant not comprising full knock-out alc genes.
2. A plant according to claim 1 , wherein one non-naturally occurring full knock-out alc gene is an ALC gene from the A genome containing a mutated splice site characterized by a G to A substitution at position 668 of SEQ ID NO: 3; and wherein one non-naturally occurring full knock-out alc gene is an ALC gene from the C genome containing a premature stopcodon characterized by a C to T substitution at position 646 of SEQ ID NO: 4.
3. A plant according to claim 1 , which is homozygous for the full knock-out alc genes.
4. A plant according to claim 1 , which produces no functional ALC protein.
5. A plant according to claim 1 , which maintains an agronomically relevant threshability of the pods.
6. A plant according to claim 1 , wherein the seed yield of the plant is increased, preferably significantly increased compared to the seed yield of a corresponding plant not comprising non-naturally occurring full knock-out alc genes.
7. A seed pod obtainable from a plant according to claim 1 .
8. A full knock-out allele of an ALC gene, wherein the ALC gene from A genome contains a mutated splice site characterized by a G to A substitution at position 668 of SEQ ID NO: 3, and wherein the ALC gene from C genome contains a premature stopcodon characterized by a C to T substitution at position 646 of SEQ ID NO: 4.
9. A method for identifying the Brassica napus plant, or a cell, part, seed or progeny thereof, of claim 1 , said method comprising identifying said two full knock-out alc genes as described in claim 1 in a biological sample comprising determining the presence of a full knock-out alc specific region of each full knock-out gene in a nucleic acid present in the biological sample.