Method for producing proteins
View Patent ↗This invention relates to a method for producing a protein of interest, comprising introducing an expression vector which comprises a gene fragment comprising a DNA encoding the protein of interest and a selectable marker gene and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between the pair of transposon sequences into a chromosome of the mammalian cell; obtaining a suspension mammalian cell producing the protein of interest; and suspension-culturing the suspension mammalian cell, and a suspension mammalian cell which expresses the protein of interest by the method.
1. A method for producing a protein of interest, comprising: introducing an expression vector which comprises a gene fragment comprising a DNA encoding the protein of interest, an attenuated selectable marker gene, and a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell;
integrating the gene fragment comprising the DNA encoding the protein of interest inserted between the pair of transposon sequences into a chromosome of the mammalian cell;
obtaining a mammalian cell which expresses the protein of interest; and
suspension-culturing the mammalian cell,
wherein the pair of transposon sequences originate from medaka fish,
wherein the attenuated selectable marker gene has a nucleotide sequence that is modified such that its expression level in the suspension mammalian cell is lowered, and wherein the attenuated selectable marker gene comprises a nucleotide sequence selected from the group consisting of SEQ ID NOs: 9, 11, 13, 21, 23, 25, 27, 29, 31 and 33.
2. A method for producing a protein of interest, comprising the following steps (A) and (B):
(A) a step of simultaneously introducing the following expression vectors (a) and (b) into a suspension mammalian cell; integrating a gene fragment inserted between a pair of transposon sequences into a chromosome of the mammalian cell by a transiently expressed transposase; and obtaining a suspension mammalian cell which expresses the protein of interest:
(a) an expression vector which comprises the gene fragment comprising a DNA encoding the protein of interest and an attenuated selectable marker gene and also comprises the pair of transposon sequences at both terminals of the gene fragment,
(b) an expression vector which comprises a DNA encoding the transposase which recognizes the transposon sequences and has activity of transferring the gene fragment inserted between the pair of transposon sequences into the chromosome,
(B) a step of suspension-culturing the suspension mammalian cell which expresses the protein of interest to produce the protein of interest,
wherein the pair of transposon sequences originate from medaka fish,
wherein the attenuated selectable marker gene has a nucleotide sequence that is modified such that its expression level in the suspension mammalian cell is lowered, and wherein the attenuated selectable marker gene comprises a nucleotide sequence selected from the group consisting of SEQ ID NOs: 9, 11, 13, 21, 23, 25, 27, 29, 31 and 33.
3. The method according to claim 1 or 2 , wherein the suspension mammalian cell is a cell capable of surviving and proliferating in a serum-free medium.
4. The method according to claim 1 or 2 , wherein the suspension mammalian cell is any one of the cells selected from the group consisting of a suspension CHO cell in which a CHO cell is adapted to suspension culture, an immortalized human embryonic retinoblast cell line, a rat myeloma cell YB⅔HL.P2.G11.16Ag.20 and a suspension mouse myeloma cell NS0 adapted to suspension culture.
5. The method according to claim 4 , wherein the CHO cell is selected from the group consisting of CHO-K1, CHO-K1SV, DUKXB11, CHO/DG44, Pro-3 and CHO-S.
6. The method according to claim 1 or 2 , wherein the pair of transposon sequences are nucleotide sequences originated from a pair of transposons which functions in a mammalian cell.
7. The method according to claim 1 or 2 , wherein the pair of transposon sequences have nucleotide sequences that are originated from a pair of Tol2.
8. The method according to claim 7 , wherein the nucleotide sequences originated from the pair of Tol2 have the nucleotide sequence of SEQ ID NO:2 and the nucleotide sequence of SEQ ID NO:3.
9. The method according to claim 1 or 2 , wherein the pair of transposon sequences have nucleotide sequences that are originated from a pair of Toll.
10. The method according to claim 9 , wherein the nucleotide sequences originated from the pair of Toll have the nucleotide sequence of SEQ ID NO:35 and the nucleotide sequence of SEQ ID NO:36.