ENHANCED IMMUNE RESPONSE IN BOVINE SPECIES
The present invention relates to a method of immune activation which is effective for eliciting a non-antigen-specific immune response in a member of the bovine species. The method is particularly effective for protecting a member of the bovine species from infectious disease and treating animals inflicted with infectious disease.
1 . An immunomodulator composition, wherein the immunomodulator composition comprises:
a. a cationic liposome delivery vehicle; and
b. a nucleic acid molecule
for treating bovine respiratory disease in cattle.
2 . The composition of claim 1 , wherein the liposome delivery vehicle comprises lipids selected from the group consisting of multilamellar vesicle lipids and extruded lipids.
3 . The composition of claims 1 and 2 , wherein the liposome delivery vehicle comprises pairs of lipids selected from the group consisting of DTMA and cholesterol; DOTAP and cholesterol; DOTIM and cholesterol, and DDAB and cholesterol.
4 . The composition of claims 1 to 3 , wherein the nucleic acid molecule is an isolated bacterially-derived nucleic acid vector without a gene insert, or a fragment thereof.
5 . The composition of claims 1 to 4 , for administration selected from the group consisting of intravenously, intramuscularly, intradermal, intraperitoneal, subcutaneously, by spray/aerosol, orally, intraocularly, intratracheally, and intranasal.
6 . The composition of claims 1 to 5 , wherein the biological agent is selected from the group consisting of an immune enhancer proteins, immunogens, vaccines, antimicrobials or any combination thereof.
7 . The composition of claim 1 , wherein the bovine respiratory disease is caused by a viral infection and/or bacterial infection.
8 . The composition according to any one of claims 1 to 7 for reducing clinical signs caused by Mannheimia haemolytica in cattle comprising an immunomodulator composition, wherein the immunomodulator composition comprises:
a. DOTIM and cholesterol lipid combination; and,
b. nucleic acid molecule is an isolated bacterially-derived nucleic acid vector without a gene insert, or a fragment thereof.
9 . The composition of claim 8 , further comprising a biologic agent.
10 . The composition according to any one of claims 1 to 9 for improving the acquired immune response of an animal that is administered a vaccine, the composition comprises an immunomodulator composition, wherein the immunomodulator composition comprises:
a. DOTIM and cholesterol lipid combination; and,
b. nucleic acid molecule is an isolated bacterially-derived nucleic acid vector without a gene insert, or a fragment thereof.
11 . The composition of claim 10 , wherein the immunomodulator composition is co-administered with the vaccine or is administered after, before, or mixed with the vaccine.