ANALYTICAL DEVICE AND ANALYTICAL METHOD
The present invention provides a technique capable of simply analyzing a target to be analyzed. An analytical device of the present invention includes a basal plate; a nucleic acid element; and a detection section of detecting a signal. The nucleic acid element and the detection section are arranged on the basal plate. The nucleic acid element includes a first nucleic acid molecule and a second nucleic acid molecule. The first nucleic acid molecule is a nucleic acid molecule capable of binding to a target. The second nucleic acid molecule is a nucleic acid molecule capable of binding to streptavidin. When the target does not bind to the first nucleic acid molecule, a binding capacity of the second nucleic acid molecule to the streptavidin is inactivated. When the target binds to the first nucleic acid molecule, a binding capacity of the second nucleic acid molecule to the streptavidin is activated. The detection section detects binding between the second nucleic acid molecule and the streptavidin. The target is bound to the first nucleic acid molecule, so that the streptavidin is bound to the second nucleic acid molecule. Thus, the target can be analyzed through detecting the binding between the second nucleic acid molecule and the streptavidin using the detection device.
1 . An analytical device comprising:
a basal plate;
a nucleic acid element; and
a detection section of detecting a signal, wherein
the nucleic acid element and the detection section are arranged on the basal plate,
the nucleic acid element comprises a first nucleic acid molecule and a second nucleic acid molecule,
the first nucleic acid molecule is a nucleic acid molecule capable of binding to a target,
the second nucleic acid molecule is a nucleic acid molecule capable of binding to streptavidin,
when the target does not bind to the first nucleic acid molecule, a binding capacity of the second nucleic acid molecule to the streptavidin is inactivated,
when the target binds to the first nucleic acid molecule, a binding capacity of the second nucleic acid molecule to the streptavidin is activated, and
the detection section detects binding between the second nucleic acid molecule and the streptavidin.
2 . The analytical device according to claim 1 , wherein
the first nucleic acid molecule is a nucleic acid molecule whose structure changes by binding of the target to the first nucleic acid molecule, and
the second nucleic acid molecule is a nucleic acid molecule whose structure changes by the change in structure of the first nucleic acid molecule.
3 . The analytical device according to claim 1 , wherein
the first nucleic acid molecule and the second nucleic acid molecule are aptamers.
4 . The analytical device according to claim 1 , wherein
the second nucleic acid molecule comprises any of the following polynucleotides (a) to (c):
(a) a polynucleotide composed of a base sequence represented by any of SEQ ID NOs: 1 to 10;
(b) a polynucleotide composed of a base sequence obtained by displacement, deletion, addition, and/or insertion of one or more bases in the base sequence (a) and capable of binding to streptavidin; and
(c) a polynucleotide composed of a base sequence having a identity of 50% or more to the base sequence (a) and capable of binding to streptavidin.
5 . The analytical device according to claim 1 , wherein
the nucleic acid element is a single-stranded nucleic acid molecule obtained by joining the first nucleic acid molecule and the second nucleic acid molecule to each other.
6 . The analytical device according to claim 1 , wherein
the nucleic acid element further comprises a linker.
7 . The analytical device according to claim 1 , wherein
streptavidin is labeled streptavidin obtained by binding a labeling substance to streptavidin, and
the signal is derived from the labeling substance.
8 . The analytical device according to claim 7 , wherein
the labeling substance emits a signal.
9 . The analytical device according to claim 7 , wherein
the labeling substance is an enzyme which catalyzes an oxidation-reduction reaction.
10 . The analytical device according to claim 9 , wherein
the labeling substance is the enzyme which catalyzes an oxidation-reduction reaction, and
the signal is emitted from a substrate by the oxidation-reduction reaction.
11 . The analytical device according to claim 10 , further comprising a reagent section, wherein
the reagent section contains a substrate for the oxidation-reduction reaction.
12 . The analytical device according to claim 7 , wherein
the signal is an electrochemical signal.
13 . The analytical device according to claim 1 , wherein
the detection section contains an electrode.
14 . The analytical device according to claim 1 , wherein
the nucleic acid element is arranged on the detection section.
15 . The analytical device according to claim 1 , comprising: two or more of the nucleic acid elements, wherein
the two or more of the nucleic acid elements each has the first nucleic acid molecule which can bind to a different target.
16 . An analytical method for analyzing a target using the analytical device according to claim 1 , the analytical method comprising:
an adding step of adding a sample and streptavidin to the analytical device; and
a detecting step of detecting binding between the second nucleic acid molecule and the streptavidin in the detection section, whereby detecting a target.
17 . The analytical method according to claim 16 , wherein
the streptavidin is labeled streptavidin obtained by binding a labeling substance to streptavidin.
18 . The analytical method according to claim 17 , wherein the labeling substance emits a signal.
19 . The analytical method according to claim 18 , wherein
the labeling substance emits a signal by an oxidation-reduction reaction.
20 . The analytical method according to claim 19 , wherein
the labeling substance is an enzyme which catalyzes an oxidation-reduction reaction.
21 . The analytical method according to claim 20 , wherein
the detecting step is performed in the presence of a substrate for the oxidation-reduction reaction.
22 . The analytical method according to claim 18 , wherein
the detection in the detecting step is detection of an electrical signal.