Isothermal nucleic acid amplification reactor with integrated solid state membrane
Provided are devices adapted to isolate, amplify, and detect nucleic acids that may be present in a biological sample. The devices can, in some embodiments, isolate, amplify, and detect nucleic acid in a single chamber. In other embodiments, the devices are adapted to isolate and amplify nucleic acids in a reaction chamber, after which the nucleic acids may be communicated to a pervious medium—such as a lateral flow strip—where the user may label and detect the nucleic acids.
1. An analysis device, comprising:
a chamber having a first and a second outlet;
a capture material that preferentially binds nucleic acids, the capture material being positioned such that the capture material is capable of fluid communication with the interior of the chamber;
a first pervious body adapted such that the first pervious body and the chamber are capable of relative motion such that the first pervious body is placed into fluid communication with the chamber;
a pervious transport material disposed at an outlet of the chamber; and
a second pervious body, the second pervious body being positionable such that the second pervious body contacts the transport material.
2. The analysis device of claim 1 , wherein the first pervious body adapted to be movably positionable such that the first pervious body contacts the capture material.
3. The analysis device of claim 1 , wherein the first and second pervious bodies are in register with the first and second outlets, respectively.
4. The analysis device of claim 1 , wherein the second pervious body comprises a binding moiety adapted to bind to a nucleic acid communicated from the transport material to the second pervious body.
5. The analysis device of claim 4 , wherein the binding moiety comprise an antibody, an antigen, a receptor, a ligand, a nucleic acid, or any combination thereof.
6. The analysis device of claim 1 , wherein the second pervious body comprises a reporter molecule adapted to associate with a nucleic acid communicated from the transport material to the second pervious body.
7. The analysis device of claim 1 , wherein the second pervious body comprises a lateral flow strip.
8. The analysis device of claim 1 , further comprising one or more quantities of fluid stored such that the fluid may be introduced to the interior of the chamber.
9. The analysis device of claim 1 , further comprising a device adapted to heat the chamber.
10. The analysis device of claim 1 , further comprising a detector capable of detecting the presence of one or more nucleic acids disposed on the second pervious body.
11. The analysis device of claim 10 , wherein the detector is exterior to the chamber.
12. The analysis device of claim 10 , wherein the detector comprises a camera, a fluorometer, or any combination thereof.
13. The analysis device of claim 1 , further comprising a quantity of fluid disposed in a container.
14. The analysis device of claim 13 , wherein the container is adapted to deliver the fluid into the chamber.
15. The analysis device of claim 13 , wherein the fluid comprises a lysis buffer, a wash buffer, water, a nucleic acid amplification agent, or any combination thereof.
16. The analysis device of claim 13 , further comprising a device configured to modulate delivery of fluid into the chamber.
17. The analysis device of claim 16 , wherein the device comprises one or more projections adapted to actuate the container so as to deliver fluid to the chamber.
18. The analysis device of claim 16 , wherein the device rotates, translates, or both, so as to actuate the container according to a preprogrammed schedule.
19. The analysis device of claim 1 , further comprising a heat-expanding material disposed so as to obstruct a passage to the chamber when the heat-expanding material is heated.
20. The device of claim 1 , further comprising a dry-stored agent, an encapsulated reagent, or both disposed within the device.
21. The device of claim 1 , wherein the second pervious body is in fluid communication with the chamber.
22. The device of claim 1 , wherein contact between the first pervious body and the capture material gives rise to a pathway capable of wicking fluid out of the chamber.
23. The device of claim 1 , wherein contact between the second pervious body and the transport material gives rise to a pathway capable of wicking amplified nucleic acid out of the chamber.