Laser beam selectors
View Patent ↗Various beam selectors for selectively placing one of at least two beams of light along the same output path are disclosed. In one aspect, a beam selector receives at least two substantially parallel beams of light. The beam selector includes a plate with an aperture so that when one of the at least two beams is selected for transmission, the beam selector directs only the selected beam along an output path through the aperture. The plate can also serve to block transmission of unselected beams. The output path through the aperture is the same for each of the at least two beams when each beam is selected. Beam selectors can be incorporated into fluorescence microscopy instruments to selectively place particular excitation beams along the same path through the microscope objective lens and into a specimen to excite fluorescence of fluorescent probes attached to a particular component of the specimen.
1. A beam selector for selecting one of at least two beams of light comprising:
a scanning mirror arranged to receive at least two beams of light;
two stationary mirrors; and
a plate with an aperture,
wherein the scanning mirror is arranged to be rotated into at least two positions,
wherein each of the at least two positions respectively selects a different one of the beams of light,
wherein the selected beam of light in each of the at least two positions undergoes a first reflection off of the scanning mirror, one reflection off of each of the stationary mirrors, and a second reflection off of the scanning mirror to place the selected beam of light on a path through the aperture, and
wherein the scanning mirror, two stationary mirrors, and plate are arranged so that a first of the stationary mirrors is angled toward a region between the scanning mirror and a second of the stationary mirrors, and the reflective surface of the second stationary mirror is angled toward a region between the scanning mirror and the first stationary mirror, and wherein each position of the at least two positions of the scanning mirror results in the selected beam to undergo a total of four reflections.
2. The beam selector of claim 1 , wherein the scanning mirror further comprises a flat mirror attached to a rotatable shaft of a motor and wherein the motor further comprises one of a galvanometer motor and a stepper motor.
3. The beam selector of claim 1 , wherein the scanning mirror further comprises a piezoelectric controlled mirror.
4. The beam selector of claim 1 , wherein each position of the at least two positions of the scanning mirror results in the selected beam to undergo a first reflection off of the scanning mirror, a second reflection off of a first of the stationary mirrors, a third reflection off of a second of the stationary mirrors, and a fourth reflection off of the scanning mirror to place the beam on the path through the aperture.
5. The beam selector of claim 1 , wherein the output path through the aperture is the same for each of the at least two excitation beams when each beam undergoes the second reflection off of the scanning mirror.
6. The beam selector of claim 1 , wherein the scanning mirror is to be rotated into each of the at least two positions in less than 0.2 milliseconds.
7. The beam selector of claim 1 , wherein the at least two beams are substantially parallel.
8. A fluorescence microscopy instrument comprising:
a parallel beam source that emits at least two substantially parallel, excitation beams of light;
a beam selector including a plate with an aperture, the selector to select only one of the at least two excitation beams for transmission on an output path through the aperture and the plate to block transmission of unselected excitation beams; and
an objective to receive the selected excitation beam and direct the beam into a specimen to excite fluorescence of fluorescent probes attached to a particular component of the specimen, and
a beam launch located in the path of the excitation beam, wherein the beam launch is positioned to receive the excitation beam and configured to attenuate the excitation beam based on an amount by which the excitation beam is to be translated,
wherein the beam selector further comprises:
a scanning mirror to receive the at least two excitation beams; and
two stationary mirrors, wherein the scanning mirror is to be rotated into at least two positions, each position to select one of the excitation beams to undergo a first reflection off of the scanning mirror, one reflection off of each of the stationary mirrors, and a second reflection off of the scanning mirror to place the excitation beam on the path through the aperture;
wherein the scanning mirror, two stationary mirrors, and the plate are arranged so that a first of the stationary mirrors is angled toward a region between the scanning mirror and a second of the stationary mirrors, and the reflective surface of the second stationary mirror is angled toward a region between the scanning mirror and the first stationary mirror.
9. The fluorescence microscopy instrument of claim 8 , wherein the parallel beam source comprises at least two lasers, each laser to emit a substantially monochromatic beam of light of a single wavelength or within a narrow range of wavelengths.
10. The fluorescence microscopy instrument of claim 9 , wherein the scanning mirror is a flat mirror attached to a rotatable shaft of a motor and wherein the motor further comprises one of a galvanometer motor and a stepper motor.
11. The fluorescence microscopy instrument of claim 9 , wherein the scanning mirror is a piezoelectric controlled mirror.
12. The fluorescence microscopy instrument of claim 9 , wherein each position of the at least two positions of the scanning mirror results in the selected beam to undergo a first reflection off of the scanning mirror, a second reflection off of a first of the stationary mirrors, a third reflection off of a second of the stationary mirrors, and a fourth reflection off of the scanning mirror to place the beam on the path through the aperture.
13. The fluorescence microscopy instrument of claim 8 , wherein the path through the aperture is the same for each of the at least two excitation beams when each beam undergoes the second reflection off of the scanning mirror.
14. The fluorescence microscopy instrument of claim 8 , wherein the beam selector includes a mirror and a motor to translate the mirror, wherein the mirror is angled to reflect the excitation beams toward the plate, and the motor is to translate the mirror so that only the selected beam is reflected along the output path.
15. The fluorescence microscopy instrument of claim 8 , wherein the beam selector includes a transparent plate and a motor to rotate the transparent plate, wherein the transparent plate is in the path of the at least two excitation beams so that the beams pass through the transparent plate toward the plate, and the motor is to rotate the transparent plate to refract the at least two excitation beams so that only the selected beam travels the output path.
16. The fluorescence microscopy instrument of claim 8 further comprising:
a dichroic mirror to reflect the selected excitation beam to the objective lens and transmit the fluorescent light;
a photodetector array; and
a lens disposed between the dichroic mirror and the photodetector, wherein the lens is to focus the fluorescent light onto the photodetector.
17. The fluorescence microscopy instrument of claim 8 , wherein the beam source emits the at least two excitation beams so that the excitation beams are substantially parallel.