Fluidic centripetal device
A fluidic centripetal apparatus for testing components of a biological material in a fluid is presented. A bottom-fillable chamber is coupled to an entry channel for receiving the fluid, the chamber inlet being provided at an outer side of the bottom-fillable chamber. A container is wholly provided in a retention chamber and contains a liquid diluent, until it releases it upon application of an external force, restoring the fluidic connection between the liquid diluent and the fluid in the retention chamber. The retention chamber can have a flow decoupling receptacle for receiving the fluid, located at the outer side of the retention chamber and interrupting a fluidic connection between the entry and exit of the retention chamber. A test apparatus and a testing method using a fluidic centripetal device for testing components of a biological material in a fluid are also provided.
1. A fluidic centripetal device for testing components of a biological material in a fluid, said fluidic centripetal device having a shape adapted to be received within a rotatable holder, said rotatable holder having a center of rotation and an outer edge, said fluidic centripetal device extending radially between said center of rotation and said outer edge, an inner side of said fluidic centripetal device being located towards said center of rotation and an outer side of said fluidic centripetal device being located towards said outer edge, the apparatus comprising:
a fluidic component layer having fluidic features on at least a front face, said fluidic features including
an entry channel for circulating said fluid, said entry channel being coupled to a chamber inlet;
a bottom-fillable chamber coupled to said entry channel at said chamber inlet for receiving said fluid, said chamber inlet being provided at an outer side of said bottom-fillable chamber, wherein said bottom-fillable chamber comprises at least one translocatable member other than beads that translocates within said bottom-fillable chamber in response to an external fluctuating magnetic field, the bottom-fillable chamber and the translocatable member being configured and dimensioned for allowing a translocation of the translocatable member within the bottom-fillable chamber while preventing exits of the translocatable member from the bottom-fillable chamber;
an exit outlet for said bottom-fillable chamber, said exit outlet allowing exit of said fluid from said bottom-fillable chamber, wherein said exit outlet is located on one longitudinal side of said bottom-fillable chamber;
an overflow chamber coupled to a surplus outlet for said bottom-fillable chamber, said surplus outlet allowing exit of part of said fluid from said bottom-fillable chamber to said overflow chamber, wherein said surplus outlet is provided near said inner side of said bottom-fillable chamber on a longitudinal side of said bottom-fillable chamber;
a vent outlet connected to a vent channel, said vent outlet being coupled to said overflow chamber for simultaneously venting said bottom-fillable chamber and said overflow chamber;
and
a bottom component layer bonded to a rear of said fluidic component layer thereby creating a fluidic network through which said fluid flows under centripetal force.
2. The device as claimed in claim 1 , further comprising an intake receptacle for receiving said fluid, said intake receptacle extending outwardly from said fluidic component layer on a front face of said fluidic component layer and being located near said inner side, said intake receptacle ending in a intake receptacle outlet, said entry channel being coupled to said intake receptacle outlet at an end opposed to said chamber inlet.
3. The device as claimed in claim 1 , wherein the bottom-fillable chamber comprises at least one object irresponsive to a fluctuating magnetic field and wherein said object is at least one of a bead, a zeolite, a particle, a filtration particle, a glass bead, a zirconium bead, a resin, a bead and resin slurry.
4. The device as claimed in claim 1 , wherein at least one of said object and said translocatable member is coated with at least one of a chelating and a ligand material adapted to interact with components of said fluid.
5. The device as claimed in claim 1 , further comprising an exit outlet for said bottom-fillable chamber, said exit outlet allowing exit of said fluid from said bottom-fillable chamber, wherein said exit outlet is located on said one longitudinal side of said bottom-fillable chamber, said exit outlet being located closer to said outer side of said bottom-fillable chamber than said surplus outlet, a metering volume of said bottom-fillable chamber being defined between said exit outlet and said surplus outlet.
6. The device as claimed in claim 5 , further comprising a burst valve at said exit outlet, said burst valve opening at a predetermined centripetal force applied on said apparatus, said burst valve preventing said fluid from exiting said bottom-Tillable chamber until said opening.
7. The device as claimed in claim 5 , further comprising a retention chamber, said retention chamber being coupled to said exit outlet at an inner side of said retention chamber, said retention chamber being located closer to said outer side of said fluidic component layer than said bottom-fillable chamber, wherein said retention chamber is coupled to said exit outlet via a metering channel, said metering channel for circulating at least a portion of said fluid from said bottom-fillable chamber to said retention chamber.
8. The device as claimed in claim 7 , further comprising a container wholly provided in said retention chamber and containing a liquid diluent, said container being adapted to maintain said liquid diluent in said container and to release said liquid diluent in said retention chamber upon application of an external force to said container, wherein said external force is one of mechanical, electrical, electromagnetic, heat, shock and acoustic force, thereby restoring the fluidic connection between said liquid diluent and said fluid in said retention chamber.
9. The device as claimed in claim 7 , wherein said retention chamber has a distribution outlet for said retention chamber, said distribution outlet being located at an outer side of said retention chamber, said distribution outlet being coupled to a transversal distribution channel at an inner side of said transversal distribution channel at a first transversal end of said distribution channel, said transversal distribution channel having a series of at least one cuvette provided at an outer side of said transversal distribution channel.
10. The device as claimed in claim 9 , wherein said at least one cuvette includes at least one of a dried reagent and a phase-change material.
11. The device as claimed in claim 9 , wherein said at least one cuvette is adapted to be optically queried for at least one parameter, said parameter is one of fluorescence, absorbance, and colorimetry.
12. The device as claimed in claim 9 , wherein said transversal distribution channel includes a waste chamber at a second transversal end of said distribution channel.
13. The device as claimed in claim 12 , wherein said waste chamber includes a phase-change material.
14. The device as claimed in claim 12 , wherein said distribution channel, said at least one cuvette and said waste chamber are provided on a portion of said fluidic layer component which extends beyond said outer edge of said rotatable holder.
15. The device as claimed in claim 14 , wherein said fluidic component layer is adapted to be divided in at least two distinct temperature-controllable sections, wherein a first of said two distinct temperature controllable sections includes at least said retention chamber and a second of said two distinct temperature controllable sections includes at least said distribution channel and said cuvettes.
16. A test apparatus using a fluidic centripetal device for testing components of a biological material in a fluid, the test apparatus comprising:
at least one of said fluidic centripetal device as claimed in claim 1 ;
a rotor assembly;
a holder for receiving said at least one of said fluidic centripetal device using said fluidic component layer, said holder being coupled to said rotor;
a motor for rotating said rotor assembly;
a speed controller for said motor for controlling at least one of a duration and a speed of rotation of said rotor assembly;
a temperature conditioning sub-system for controlling a temperature of at least a portion of said micro-fluidic centripetal device;
a detection sub-system for detecting a characteristic of said fluid; and
a user interface for receiving a user command and for sending a command to at least one of said speed controller, said temperature conditioning sub-system, said excitation sub-system and said detection sub-system.
17. The test apparatus as claimed in claim 16 , wherein said temperature conditioning sub-system controls a temperature of at least two zones of said fluidic centripetal device.
18. A testing method using a fluidic centripetal device for testing components of a biological material in a fluid, the method comprising:
providing at least one of said fluidic centripetal device as claimed in claim 1 ;
providing a test apparatus as claimed in claim 16 ;
providing a fluid with biological material;
loading said fluid in said intake receptacle of said fluidic centripetal device;
placing said fluidic centripetal device in said holder of said test apparatus;
providing a user command to commence a test sequence;
rotating said rotor assembly at a first speed to transfer said fluid from said intake receptacle to said bottom-Tillable chamber.
19. The testing method of claim 18 , wherein the fluid is selected from the group consisting of blood, nasal pharyngeal aspiration, oral fluid, liquid from resuspended oral swab, liquid from resuspended nasal swab, liquid resuspended from anal swab, liquid resuspended from vaginal swab, saliva and urine.
20. The testing method of claim 18 , for testing at least one component selected from the group consisting of ions, sugars, metabolites, fatty acids, amino acids, nucleic acids, proteins and lipids.