Arrangement for preservation of biological samples
View Patent ↗The present invention generally relates to systems and methods for preserving biological samples and, more particularly, an arrangement for drying biological samples disposed on sample storage substrates. The systems and methods for preserving biological samples can include a heating element such as a chemical heating pad that facilitates drying of the biological samples.
1. An arrangement for drying of a biological sample applied on a sample storage substrate, comprising:
at least one sample storage substrate comprising a front side for receiving the biological sample and a back side, the sample storage substrate being formed of a paper material;
at least one sample storage substrate holder;
at least one chemical heat pad positioned below the back side of the sample storage substrate;
a chemical heat pad holder that is hinged and moveable towards the sample storage substrate so as to position the chemical heat pad in proximity of the sample storage substrate; and
a protrusion, wherein said protrusion is configured to activate said chemical heat pad to accelerate the drying of the biological sample on the sample storage substrate,
wherein a ceiling surface temperature of said chemical heat pad is between 35 and 58° C., and
wherein the sample storage substrate holder comprises one or more air passageways, the one or more air passageways being arranged to expose the sample storage substrate to air outside the sample storage substrate holder when the sample storage substrate holder is in a closed configuration.
2. The arrangement of claim 1 , wherein said sample storage substrate comprises a sheet of porous material.
3. The arrangement of claim 1 , wherein said sample storage substrate further comprises at least one reagent for stabilization of components in said biological sample or for lysis of cells in said sample.
4. The arrangement of claim 1 , wherein said chemical heat pad is configured to maintain a surface temperature within the 35-58° C. interval for at least 10 min.
5. The arrangement of claim 1 , wherein the chemical heat pad is positioned at a distance of less than about 10 mm from said sample storage substrate.
6. The arrangement of claim 1 , wherein the chemical heat pad is positioned in contact with said sample storage substrate.
7. The arrangement of claim 1 , wherein said protrusion is arranged to act upon a slit metal disk or spring in said chemical heat pad.
8. The arrangement of claim 1 , further comprising a moveable sample collection member hinged and configured to move between a first open position for collecting the biological sample on the analyte collection surface and a second closed position facing or contacting at least a portion of the sample storage substrate, wherein said sample collection member has an analyte collection surface.
9. The arrangement of claim 1 , further comprising a housing with a plurality of said sample storage substrates and wherein said housing comprises air vents in connection with air channels between the sample storage substrates.
10. The arrangement of claim 1 , wherein said sample storage substrate comprises at least one reagent for lysis of cells in said sample.
11. The arrangement of claim 1 , wherein said sample storage substrate comprises a surfactant or a chaotrope.
12. The arrangement of claim 1 , wherein said sample storage substrate comprises an anionic surfactant, a guanidinium salt or urea.
13. The arrangement of claim 1 , wherein the sample storage substrate is positioned perpendicularly to a length axis of the heat pad.
14. The arrangement of claim 1 , further comprising a protective film between the chemical heat pad and the sample storage substrate.