Granted Patent B2

US 9,758,834 · App. 14/007,586 · Granted Sep 12, 2017

Abstract

Claims (27)

1. A composition for the diagnosis of leukemia comprising a probe pair comprising a first probe and a second probe,

said first probe comprising a complex comprising:

(a) a first molecule, comprising:

(i) a first label attachment region to which are attached one or more label monomers that emit light constituting a first signal;

(ii) a second label attachment region, which is non-overlapping with the first label attachment region, to which are attached one or more label monomers that emit light constituting a second signal; and

(b) a first target-specific sequence attached to the first molecule,

wherein at least one of the first probes comprises a first target-specific sequence comprising a sequence selected from the group consisting of SEQ ID NO: 139, 142, 145, 148, 151, 154, and 157,

said second probe comprising a second molecule, comprising

(i) a second target-specific sequence,

wherein at least one of the second probes comprises a second target-specific sequence comprising a sequence selected from the group consisting of SEQ ID NO: 140, 143, 146, 149, 152, 155, and 158; and

(ii) an affinity tag, wherein the affinity tag is biotin;

wherein the first target-specific sequence and the second target-specific sequence bind to different regions of the same target molecule,

wherein the target molecule is a fusion gene or mRNA transcribed from said fusion gene,

wherein the fusion gene is the result of the fusion of two or more genes in a patient at the onset of or during progression of leukemia and wherein when said probe pair is bound to its target molecule, the identity of the first and second signals and their locations relative to each other constitute at least part of a code that identifies the target molecule,

wherein the fusion gene comprises the fusion of at least a portion of a first gene and at least a portion of a second gene, and

wherein the first target-specific sequence hybridizes to a portion of the first gene and the second target-specific sequence hybridizes to a portion of the second gene.

2. The composition of claim 1 , wherein the first target-specific sequence hybridizes to a sequence adjacent to a sequence that the second target-specific sequence hybridizes to on the fusion gene.

3. The composition of claim 1 , wherein the first target-specific sequence hybridizes to a portion of the first gene and a portion of the second gene.

4. The composition of claim 3 , wherein the first target-specific sequence hybridizes to a sequence adjacent to a sequence that the second target-specific sequence hybridizes to on the fusion gene.

5. The composition of claim 1 , wherein the second target-specific sequence hybridizes to a portion of the first gene and a portion of the second gene.

6. The composition of claim 5 , wherein the first target-specific sequence hybridizes to a sequence adjacent to a sequence that the second target-specific sequence hybridizes to on the fusion gene.

7. The composition of claim 5 , wherein at least one of the target-specific sequences comprises a region with one or more mismatches to the target of the target-specific sequence.

8. The composition of claim 1 , wherein the leukemia is selected from the group consisting of chronic myelogenous leukemia, acute myeloid leukemia and acute lymphoblastic leukemia.

9. The composition of claim 1 , wherein each of said label monomers are selected from the group consisting of a fluorochrome moiety, a fluorescent moiety, a dye moiety or a chemiluminescent moiety.

10. The composition of claim 9 , wherein each of said label monomers comprise a fluorescent moiety.

11. The composition of claim 9 , wherein the unique label for each target-specific probe is composed of 4 label monomers.

12. The composition of claim 9 , wherein the unique label for each target-specific probe is composed of 5 label monomers.

Assignments (9)