Conditioned medium and extracellular matrix compositions from cells cultured under hypoxic conditions for methods of treating tissue injury
The present invention is directed to a method of producing compositions including embryonic proteins. The method includes culturing cells under hypoxic conditions on a biocompatible surface in vitro. The culturing method produces both soluble and non-soluble fractions, which may be used separately or in combination to obtain physiologically acceptable compositions useful in a variety of medical and therapeutic applications.
1. A method of producing a composition for treating tissue in need of repair comprising:
culturing fibroblast cells under hypoxic conditions on a microcarrier bead surface in a suitable growth medium, under 1-5% oxygen for at least 2 weeks, thereby producing multipotent stem cells, wherein the multipotent stem cells produce and secrete a composition that promotes repair and regeneration of damaged tissue when administered to the region of tissue in need of repair in a subject and collecting the growth medium after the 2 weeks, thereby producing the composition.
2. A composition produced by the method of claim 1 .
3. A method of treating damaged tissue comprising contacting the damaged tissue with a composition made by the method of claim 1 under conditions that allow for treatment of the damaged tissue.
4. The method of claim 1 , wherein the tissue is connective tissue.
5. The method of claim 3 , wherein the tissue is connective tissue.
6. The method of claim 4 , wherein the tissue is bone, cartilage, ligament, tendon, meniscus, dermis, hyperdermis, muscle, fatty tissue, joint capsule, intestinal or heart tissue.
7. The method of claim 5 , wherein the tissue is bone, cartilage, ligament, tendon, meniscus, dermis, hyperdermis, muscle, fatty tissue, joint capsule, intestinal or heart tissue.
8. The method of claim 6 , wherein the tissue is infarcted or ischemic tissue.
9. The method of claim 7 , wherein the tissue is infarcted or ischemic tissue.
10. A method for improvement of a skin surface in a subject comprising administering to the subject at the site of a wrinkle or scar, a composition made by the method of claim 1 , thereby providing an improved skin surface.
11. A method for soft tissue repair or augmentation in a subject comprising administering to the subject at the site in need of repair, a composition made by the method of claim 1 , thereby providing soft tissue repair or augmentation.