Process to avoid inhibition of acetogens by CO
View Patent ↗A process is provided for fermenting CO-containing gaseous substrates. The process is effective for decreasing lag times and maintaining a culture in steady state by controlling CO concentration and minimizing effects of high or low CO concentrations during fermentation. The process includes providing syngas to a first fermentation zone, fermenting the syngas, and determining a CO concentration in a fermentation medium in the first fermentation zone. If the CO concentration in fermentation medium in the first fermentation zone has a calculated value of about 0.12 mM or greater, then at least a portion of the syngas being provided to the first fermentation zone is provided to one or more subsequent fermentation zones in an amount effective for providing a calculated CO concentration in any subsequent fermentation zone of about 0.12 mM or less.
1. A method for reducing growth inhibition by carbon monoxide (CO) of microbes in fermentation, comprising:
a) providing syngas to a first fermentation zone;
b) fermenting the syngas; and
c) determining the CO concentration in the fermentation medium in the first fermentation zone, wherein if the CO concentration in the fermentation medium in the first fermentation zone has a calculated value of 0.12 mM or greater, then at least a portion of the syngas being provided to the first fermentation zone is provided to one or more subsequent fermentation zones in an amount effective for providing a calculated CO concentration in the first fermentation zone of at least 0.12 mM or less.
2. The process of claim 1 wherein CO concentration is calculated by determining a supply factor, wherein the supply factor=(head space CO %÷100)×(((measured reactor pressure (psig)+atmospheric pressure))÷(atmospheric pressure×14.7)).
3. The process of claim 1 wherein the syngas provided to the first or any subsequent fermentation zone has a molar ratio of H 2 to CO of 0.2 or more.
4. The process of claim 1 wherein the syngas provided to the first or any subsequent fermentation zone has 40 mole percent or more of CO plus H 2 and an H 2 content of 30 mole percent or less.
5. The process of claim 1 wherein the syngas provided to the first or any subsequent fermentation zone has a CO/CO 2 molar ratio of at least 0.75.
6. The process of claim 1 wherein the process is effective for increasing cell density to 2.0 g/L or more in the first or any subsequent fermentation zone.
7. The process of claim 1 wherein the process provides a calculated CO concentration to initial cell density ratio of about 0.5 to about 0.9.
8. The process of claim 1 wherein the fermentation medium includes microbes capable of consuming and/or producing CO.
9. The process of claim 8 wherein the microbes are acetogenic bacteria.
10. The process of claim 9 wherein the acetogenic bacteria are selected from the group consisting of Acetogenium kivui, Acetoanaerobium noterae, Acetobacterium woodii, Alkalibaculum bacchi CP11 (ATCC BAA-1772), Blautia producta, Butyribacterium methytotrophicum, Caldanaerobacter subterraneous, Caldanaerobacter subterraneous pacificus, Carboxydothermus hydrogenoformans, Clostridium aceticum, Clostridium acetobutylieum, Clostridium acetobutylicum P262 , Clostridium autoethanogenum, Clostridium autoethanogenum (DSM 10061 of DSMZ Germany), Clostridium autoethanogenum (DSM 23693 of DSMZ Germany), Clostridium autoethanogenum (DSM 24138 of DSMZ Germany), Ctostridium carboxidivorans P7 (ATCC PTA-7827), Clostridium coskatii (ATCC PTA-10522), Ctostridium drakei, Clostridium ljungdahlii PETC (ATCC 49587), Clostridium ljungdahlii ERI2 (ATCC 55380), Clostridium ljungdahlii C-01 (ATCC 55988), Clostridium ljungdahlii O-52 (ATCC 55889), Clostridium magnum, Clostridium pasteurianum (DSM 525 of DSMZ Germany), Clostridium ragsdali P11 (ATCC BAA-622), Clostridium scatologenes, Ctostridium thermoaceticum, Ctostridium ultunense, Desulfotomaculum kuznetsovii, Eubacterium limosum, Geobacter sulfurreducens, Methanosarcina acetivorans, Methanosarcina barkeri, Morrella thermoaeetica, Morrella thermoautotrophica, Oxobacter pfennigii, Peptostreptococcus productus, Ruminococcus productus, Thermoanaerobacter kivui and mixtures thereof.
11. A method for reducing growth inhibition by carbon monoxide (CO) of microbes in fermentation, comprising:
a) providing syngas to a first fermentor;
b) fermenting the syngas; and
c) determining the CO concentration in the fermentation medium in the first fermentor, wherein if the CO concentration in the fermentation medium in the first fermentor has a calculated value of 0.12 mM or greater, then at least a portion of the syngas being provided to the first fermentor is provided to one or more subsequent fermentors in an amount effective for providing a calculated CO concentration in the first fermentor of at least 0.12 mM or less.
12. The process of claim 11 wherein CO concentration is calculated by determining a supply factor, wherein the supply factor=(head space CO %÷100)×(((measured reactor pressure (psig)+atmospheric pressure))÷(atmospheric pressure×14.7)).
13. The process of claim 11 wherein the syngas provided to the first or any subsequent fermentation zone has a molar ratio of H 2 to CO of 0.2 or more.
14. The process of claim 11 wherein the syngas provided to the first or any subsequent fermentation zone has 40 mole percent or more of CO plus H 2 and an H 2 content of 30 mole percent or less.
15. The process of claim 11 wherein the syngas provided to the first or any subsequent fermentation zone has a CO/OO 2 molar ratio of at least 0.75.
16. The process of claim 11 wherein the process is effective for increasing cell density to 2.0 g/L or more in the first or any subsequent fermentation zone.
17. The process of claim 11 wherein the process is effective for maintaining a calculated CO concentration to cell density ratio of about 0.001 to about 1.0.
18. The process of claim 11 wherein the fermentation medium includes microbes capable of consuming and/or producing CO.
19. The process of claim 18 wherein the microbes are acetogenic bacteria.
20. The process of claim 19 wherein the acetogenic bacteria are selected from the group consisting of Acetogenium kivui, Acetoanaerobium noterae, Acetobacterium woodii, Alkalibaculum bacchi CP11 (ATCC BAA-1772), Blautia producta, Butyribacterium methytotrophicum, Caldanaerobacter subterraneous, Caldanaerobacter subterraneous pacificus, Carboxydothermus hydrogenoformans, Clostridium aceticum, Clostridium acetobutylieum, Clostridium acetobutylicum P262 , Clostridium autoethanogenum, Clostridium autoethanogenum (DSM 10061 of DSMZ Germany), Clostridium autoethanogenum (DSM 23693 of DSMZ Germany), Clostridium autoethanogenum (DSM 24138 of DSMZ Germany), Ctostridium carboxidivorans P7 (ATCC PTA-7827), Clostridium coskatii (ATCC PTA-10522), Ctostridium drakei, Clostridium ljungdahlii PETC (ATCC 49587), Clostridium ljungdahlii ERI2 (ATCC 55380), Clostridium ljungdahlii C-01 (ATCC 55988), Clostridium ljungdahlii O-52 (ATCC 55889), Clostridium magnum, Clostridium pasteurianum (DSM 525 of DSMZ Germany), Clostridium ragsdali P11 (ATCC BAA-622), Clostridium scatologenes, Ctostridium thermoaceticum, Ctostridium ultunense, Desulfotomaculum kuznetsovii, Eubacterium limosum, Geobacter sulfurreducens, Methanosarcina acetivorans, Methanosarcina barkeri, Morrella thermoaeetica, Morrella thermoautotrophica, Oxobacter pfennigii, Peptostreptococcus productus, Ruminococcus productus, Thermoanaerobacter kivui and mixtures thereof.
21. The process of claim 11 wherein syngas is provided to subsequent fermentors operating in parallel.
22. The process of claim 11 wherein syngas is provided to subsequent fermentors operating in series.
23. The process of claim 11 wherein the first or subsequent fermentors are different fermentation zones in the fermentor.
24. The process of claim 11 wherein at least a portion of off-gas from a first or any subsequent fermentor is provided to a vent-gas boiler.
25. A method for reducing growth inhibition by carbon monoxide (CO) of microbes in fermentation, comprising
a) providing syngas to a fermentation medium in an amount effective for providing an initial calculated CO concentration in the fermentation medium of about 0.15 mM to about 0.70 mM, wherein the method is effective for increasing cell density of the microbes as compared to the same method in which the concentration of CO is not controlled.