Process to control CO concentrations in fermentations
View Patent ↗A process for controlling concentration of CO in a bioreactor provides a direct and real time measurement of dissolved CO in a fermentation medium. The process for controlling concentrations of CO in a bioreactor includes contacting an aliquot of fermentation medium with at least one CO binding ligand and at least one microbial inactivator.
1. A process for controlling the concentration of carbon monoxide (CO), in an aqueous fermentation medium comprising CO, the process comprising:
a) combining part of the fermentation medium with at least one CO binding ligand and
b) measuring the amount of CO bound to the CO binding ligand, wherein at least a portion of the CO is bound to the CO binding ligand.
2. The process of claim 1 wherein part of the fermentation medium is combined with the CO binding ligand and with a microbial inactivator.
3. The process of claim 1 wherein the CO concentration in the aqueous fermentation medium is maintained from about 2 μM or more to about 10 μM or less.
4. The process of claim 1 wherein the CO bonding ligand is selected from the group consisting of hemoglobin, myoglobin, free heme, heme containing compounds and mixtures thereof.
5. The process of claim 2 wherein the microbial inactivator is selected from the group consisting of salt, acid, base, organic solvent, oxidizing agents, heat, cold and mixtures thereof.
6. The process of claim 1 wherein the amount of CO bound to the CO binding ligand is measured by determining changes in absorbance.
7. A process for controlling the concentration of CO in a bioreactor that includes an aqueous fermentation medium, the process comprising:
a) combining an aliquot of fermentation medium with at least one CO binding ligand and at least one microbial inactivator; and
b) measuring the amount of CO bound to the CO binding ligand, wherein at least a portion of the CO is bound to the CO binding ligand.
8. The process of claim 7 , further comprising decreasing the feed rate of CO to the bioreactor when if the amount of CO bound to the CO binding ligand corresponds to a concentration of CO in the fermentation medium of 10 μM or more.
9. The process of claim 7 , further comprising increasing the feed rate of CO to the bioreactor when if the amount of CO bound to the CO binding ligand corresponds to a concentration of CO in the fermentation medium of 2 μM or less.
10. The process of claim 7 wherein the aqueous fermentation medium includes microbes effective for consuming and/or producing CO.
11. The process of claim 7 wherein the CO bonding ligand is selected from the group consisting of hemoglobin, myoglobin, free heme, heme containing compounds and mixtures thereof.
12. The process of claim 7 wherein the microbial inactivator is selected from the group consisting of salt, acid, base, organic solvent, oxidizing agents, heat, cold and mixtures thereof.
13. The process of claim 7 wherein the amount of CO bound to the CO binding ligand is measured by determining changes in absorbance.
14. The process of claim 10 wherein the microbes are acetogenic bacteria.
15. The process of claim 14 wherein the acetogenic bacteria are selected from the group consisting of Acetogenium kivui, Acetoanaerobium noterae, Acetobacterium woodii, Alkalibaculum bacchi CP11 (ATCC BAA-1772), Blautia producta, Butyribacterium methytotrophicum, Caldanaerobacter subterraneous, Caldanaerobacter subterraneous pacificus, Carboxydothermus hydrogenoformans, Clostridium aceticum, Clostridium acetobutylieum, Clostridium acetobutylicum P262 , Clostridium autoethanogenum, Clostridium autoethanogenum (DSM 10061 of DSMZ Germany), Clostridium autoethanogenum (DSM 23693 of DSMZ Germany), Clostridium autoethanogenum (DSM 24138 of DSMZ Germany), Clostridium carboxidivorans P7 (ATCC PTA-7827), Clostridium coskatii (ATCC PTA-10522), Clostridium drakei, Clostridium ljungdahlii PETC (ATCC 49587), Clostridium ljungdahlii ERI2 (ATCC 55380), Clostridium ljungdahlii C-01 (ATCC 55988), Clostridium ljungdahlii O-52 (ATCC 55889), Clostridium magnum, Clostridium pasteurianum (DSM 525 of DSMZ Germany), Clostridium ragsdali P11 (ATCC BAA-622), Clostridium scatologenes, Clostridium thermoaceticum, Clostridium ultunense, Desulfotomaculum kuznetsovii, Eubacterium limosum, Geobacter sulfurreducens, Methanosarcina acetivorans, Methanosarcina barkeri, Morrella thermoaeetica, Morrella thermoautotrophica, Oxobacter pfennigii, Peptostreptococcus productus, Ruminococcus productus, Thermoanaerobacter kivui and mixtures thereof.