Cell Preparations For Extemporaneous Use, Useful for Healing and Rejuvenation In Vivo
The present invention relates to new plasma or new platelet-rich plasma preparations, new cell dissociation methods, new cell associations or compositions, a method of preparation thereof, a use thereof, devices for the preparation thereof and preparations containing such a platelet-rich plasma preparation and cell associations or compositions. Specifically, the invention provides plasma or platelet-rich plasma alone or in cell combinasons preparations for use in tissue regeneration and bone regeneration and pain reduction.
1 . An isolated cell composition comprising:
a) plasma;
b) platelets at a concentration of at least 300×10 9 cells/L;
c) white blood cells at a concentration of at least 7.0×10 9 cells/L; and
d) fibrinogen at a concentration of at least 3 mg/L;
and wherein the erythrocyte concentration is less than 0.6×10 12 cells/L.
2 . An implantable device for use in tissue regeneration therapy comprising:
a) a permeable core comprising a cell composition comprising:
i) plasma;
ii) platelets at a concentration of at least 300×10 9 cells/L;
iii) white blood cells at a concentration of at least 7.0×10 9 cells/L;
iv) fibrinogen at a concentration of at least 3 mg/L; and
v) a cell extract where cells are selected from dermal cells, keratinocytes, fibroblasts, melanocytes and Langheran's cells; fat cells; bone marrow cells; muscle cells; osteoblasts; chondrocytes; periosteal membrane cells; corneal cells; umbilical cord cells; Schwann cells, tendon cells and pancreas islet cells, wherein cells are at a concentration of about 10 5 to about 10 6 cells/L and wherein the erythrocyte concentration of said cell composition is less than 0.6×10 12 cells/L; and
b) an external jacket surrounding said core, said jacket comprising a biocompatible material or a bioresorbable material.
3 . A device for the preparation of a platelet concentrate from whole blood comprising a separator tube wherein the separator tube is selected from:
a glass separator tube containing a polyester-based thixotropic gel and a buffered sodium citrate solution at 0.10 M; and
a polyethylene terephthalate separator tube containing a highly thixotropic gel formed by a polymer mixture and an anhydrous sodium citrate at 3.5 mg/mL;
characterised in that the device has an inlet for introducing said whole blood, is held in a vacuum intended to aspirate the whole blood sample, is sterile, has a usable vacuum of or about 8 to about 10 mL and is suitable for undergoing centrifugation.