Lateral flow assays for tagged analytes
View Patent ↗Methods of determining whether an analyte is present in a sample using a competitive assay are provided. Aspects of the methods include employing a competitor that provides for the obtainment of signal which is directly proportional to the amount of analyte in the sample. Also provided are devices and kits that find use in practicing the methods described herein.
1. A method of determining whether an analyte is present in a sample, the method comprising:
(a) providing a sample-contacted sample receiving region of a test lateral flow assay device, wherein the sample-contacted sample receiving region comprises:
(i) a quantity of the sample;
(ii) an immobilized first capture probe that specifically binds to the analyte; and
(iii) a competitor comprising a first binding pair member that specifically binds to the first capture probe; and
(b) reading a detection region of the test lateral flow assay device to determine whether the analyte is present in the sample, wherein the detection region comprises an immobilized detection capture probe that specifically binds to the competitor.
2. The method according to claim 1 , wherein the sample-contacted sample receiving region is provided by combining the sample with the competitor prior to applying the sample to the sample receiving region.
3. The method according to claim 1 , wherein the sample-contacted sample receiving region is provided by applying the competitor to the sample receiving region prior to applying the sample to the sample receiving region.
4. The method according to claim 1 , wherein the sample-contacted sample receiving region is provided by applying the sample to the sample receiving region prior to applying the competitor to the sample receiving region.
5. The method according to claim 1 , wherein the sample receiving region comprises the competitor.
6. The method according to claim 1 , wherein the competitor further comprises a second binding pair member that specifically binds to the detection capture probe.
7. The method according to claim 1 , wherein the competitor further comprises a label moiety.
8. The method according to claim 1 , wherein the test lateral flow assay device further comprises an internal control region downstream from the detection region.
9. The method according to claim 1 , wherein the sample-contacted sample receiving region of the test lateral flow assay device further comprises a reporter that specifically binds to the competitor.
10. The method according to claim 1 , wherein the first binding pair member is a tag and the analyte is a tagged analyte.
11. The method according to claim 10 , the method further comprising: producing the sample comprising the tagged analyte.
12. The method according to claim 1 , the method further comprising:
applying a control sample to a sample receiving region of a control lateral flow assay device; and
reading a detection region of the control lateral flow assay device, wherein the control lateral flow assay device is identical to the test lateral flow assay device.