Processes and systems for the production of fermentation products
View Patent ↗The present invention relates to the production of fermentation products such as alcohols including ethanol and butanol, and processes employing in situ product removal methods.
1. A method for producing and recovering a product alcohol from a fermentation broth comprising:
(a) providing a feedstock slurry comprising fermentable carbon source, undissolved solids, oil, and water;
(b) separating the feedstock slurry by three-phase centrifugation to generate (i) an aqueous solution comprising fermentable carbon source, (ii) a wet cake comprising solids, and (iii) an oil stream;
(c) adding the aqueous solution to a fermentation broth in a fermentor; wherein the fermentation broth comprises a microorganism that produces a product alcohol and whereby a product alcohol is produced;
(d) conducting the fermentation broth of (c) to one or more external extractors, wherein the fermentation broth is mixed with an extractant forming an aqueous phase and an extractant phase and wherein the extractant phase is maintained as a continuous phase; and
(e) recovering the product alcohol.
2. The method of claim 1 , wherein the one or more external extractors is one or more siphons, decanters, centrifuges, gravity settlers, phase splitters, mixer-settlers, column extractors, centrifugal extractors, agitated extractors, hydrocyclones, spray towers, or combinations thereof.
3. The method of claim 1 , wherein the product alcohol is transferred to the extractant phase.
4. The method of claim 3 , wherein the product alcohol is recovered from the extractant phase by distillation.
5. The method of claim 1 , wherein a portion of the extractant phase is returned to the extractor as reflux.
6. The method of claim 1 , wherein the fermentation broth is conducted to the one or more external extractors via a distributor or dispersal device.
7. The method of claim 1 , wherein the one or more external extractors comprise internals, exit ports for carbon dioxide, coalescing pads, or combinations thereof.
8. The method of claim 1 , wherein the one or more external extractors comprise a conical shape.
9. The method of claim 1 , wherein the one or more external extractors comprise a first region of constant diameter and a stepwise increase of diameter to a second region of constant diameter.
10. The method of claim 9 , wherein the one or more external extractors further comprise a second stepwise increase of diameter to a third region of constant diameter.
11. The method of claim 1 , wherein a heterogeneous mixture comprising the fermentation broth and the extractant is further processed through one or more hydrocyclones.
12. The method of claim 1 , wherein the oil stream is hydrolyzed by one or more enzymes to form fatty acids.
13. The method of claim 12 , wherein the fatty acids are added to the fermentation broth as the extractant.
14. The method of claim 12 , wherein the one or more enzymes is one or more lipases or phospholipases.
15. The method of claim 1 , wherein the product alcohol is ethanol, propanol, butanol, pentanol, hexanol, fusel alcohols, or mixtures thereof.
16. The method of claim 1 , wherein the microorganism comprises a butanol biosynthetic pathway.
17. The method of claim 16 , wherein the butanol biosynthetic pathway is a 1-butanol biosynthetic pathway, a 2-butanol biosynthetic pathway, or an isobutanol biosynthetic pathway.