Method and device for depositing a substance on a submerged surface
The present disclosure provides apparatuses, systems, and methods involving a spotter for depositing a substance on a submerged surface. The spotter comprises an outlet cavity defined at least in part by a spotting orifice, a first opening, and a second opening. The spotter also comprises a first conduit fluidly coupled to the first opening and a second conduit fluidly coupled to the second opening. The spotter is adapted so that fluid flowing through the first conduit and the second conduit is communicated among the first opening, the second opening, and a submerged deposition surface when the sealing orifice is sealed against the submerged deposition surface to form a deposition spot on the submerged deposition surface. The submerged deposition surface is within a liquid such that the liquid covers the deposition spot upon removal of the orifice from the deposition surface.
1. A method for depositing a substance on a submerged deposition surface, comprising:
obtaining a spotter, including:
an outlet cavity defined at least in part by a spotting orifice, a first opening, and a second opening,
a first conduit fluidly coupled to the first opening, and
a second conduit fluidly coupled to the second opening;
sealing the spotting orifice against a deposition surface;
causing a fluid comprising a deposition material to flow through the first conduit into the outlet cavity and out through the second conduit, thereby communicating among the first opening, the second opening, and the deposition surface to form a deposition spot comprising the deposition material on the deposition surface via the spotting orifice sealed against the deposition surface; and
removing the spotting orifice from the deposition surface, wherein the deposition surface is submerged and the spotter is at least partially submerged in a liquid prior to removing the spotting orifice from the deposition surface such that the liquid covers the deposition spot when the spotting orifice is removed from the deposition surface, wherein the liquid is not the fluid comprising the deposition material.
2. The method of claim 1 , wherein the deposition surface is submerged in the liquid in a liquid reservoir.
3. The method of claim 2 , wherein the liquid reservoir forms at least a portion of a microtiter plate.
4. The method of claim 1 , wherein the deposition surface forms at least a portion of a liquid reservoir.
5. The method of claim 2 , further comprising delivering the liquid to the liquid reservoir.
6. The method of claim 5 , wherein the liquid is delivered to the liquid reservoir prior to sealing the spotting orifice against the deposition surface.
7. The method of claim 5 , wherein the liquid is delivered to the liquid reservoir after sealing the spotting orifice against the deposition surface and prior to removing the spotting orifice from the deposition surface.
8. The method of claim 5 , wherein the liquid is delivered in a predetermined amount.
9. The method of claim 2 , wherein the outlet cavity comprises a plurality of outlet cavities with a plurality of spotting orifices, and the first conduit and the second conduit comprise a plurality of first and second conduits fluidly coupled to first and second openings, respectively, of the plurality of outlet cavities.
10. The method of claim 9 , wherein the deposition surface comprises a plurality of deposition surfaces, and the liquid reservoir comprises a plurality of wells of a microtiter plate.
11. The method of claim 10 , wherein each orifice of the plurality of spotting orifices is configured to seal against a different one of the plurality of deposition surfaces of the plurality of wells.
12. The method of claim 1 , wherein the liquid comprises a solution.
13. The method of claim 1 , further comprising orienting the deposition surface in a horizontal orientation.
14. The method of claim 1 , further comprising sensing a force of the spotting orifice against the deposition surface to facilitate sealing the spotting orifice against the deposition surface.
15. The method of claim 1 , further comprising controlling, with a control system, sealing of the spotting orifice against the deposition surface and causing fluid to flow through the first conduit and the second conduit.
16. The method of claim 1 , further comprising driving the fluid back and forth through the first and second conduits.
17. The method of claim 1 , wherein the liquid is present on the deposition surface before the spotting orifice is sealed against the deposition surface.