Monoclonal antibodies with specificity for fetal erythroid cells
The present invention concerns a monoclonal antibody and corresponding hybridoma cells and antigens suitable for isolating fetal cells from maternal blood. The inventive monoclonal antibody reacts with a surface antigen present on fetal red blood cells including their nucleated precursor cells, but not with surface antigens on adult erythroid cell.
1. A method for detecting whether fetal red blood cells or their nucleated precursor cells are present in a sample comprising:
(a) contacting a sample with a fluorescently labeled antibody that binds to the surface of fetal red blood cells and their nucleated precursor cells, wherein said antibody is not reactive with CD71 or with surface antigens of adult erythroid, lymphatic, or myeloic cells, under conditions in which the antibody binds to fetal red blood cells and their nucleated precursor cells if present in the sample; and
(b) detecting cells bound by said fluorescently labeled antibody if present in the sample, wherein binding of the antibody indicates that the cell is a fetal red blood cell or a nucleated precursor cell thereof.
2. The method according to claim 1 , wherein fetal red blood cells or their nucleated precursor cells are present in the sample and wherein step (b) comprises detecting cells bound by said fluorescently labeled antibody.
3. The method according to claim 2 , further comprising analyzing cells bound by said fluorescently labeled antibody for a chromosomal or genetic defect.
4. The method according to claim 3 , wherein the antibody is a monoclonal antibody.
5. The method according to claim 4 , wherein the sample is maternal blood.
6. The method according to claim 3 , wherein the sample is maternal blood.
7. The method according to claim 2 , wherein the antibody is a monoclonal antibody.
8. The method according to claim 7 , wherein the sample is maternal blood.
9. The method according to claim 2 , wherein the sample is maternal blood.
10. The method according to claim 1 , which comprises separating cells bound by said fluorescently labeled antibody by flow cytometry, solid phase separation, immunomagnetic bead separation, or panning on plastic surfaces.
11. The method according to claim 10 , wherein the antibody is a monoclonal antibody.
12. The method according to claim 11 , wherein the sample is maternal blood.
13. The method according to claim 10 , wherein the sample is maternal blood.
14. The method according to claim 1 , wherein the antibody is a monoclonal antibody.
15. The method according to claim 14 , wherein the sample is maternal blood.
16. The method according to claim 1 , wherein the sample is maternal blood.