Method for producing polymer, method for producing organic acid, and organic acid-producing microorganism
View Patent ↗The present invention provides a method of producing a polymer, which comprises the step of performing a polymerization reaction using, as a starting material, an organic acid obtained by allowing a microorganism or a treated cell thereof to act on an organic raw material, wherein said microorganism has an ability to produce an organic acid and has been modified so as to produce less aromatic carboxylic acid as compared to an unmodified strain.
1. A Coryneform bacterium, which has an ability to produce an organic acid and has been modified so that at least one enzyme activity selected from the group consisting of dehydroquinate synthase activity, dehydroquinate dehydratase activity and dehydroshikimate dehydratase activity is reduced as compared to an unmodified strain, and the production of an aromatic carboxylic acid from a fermentable sugar is thereby reduced, wherein the at least one enzyme activity is reduced by disrupting a gene encoding the at least one enzyme, mutating a gene encoding the at least one enzyme, or modifying an expression control sequence of a gene encoding the at least one enzyme.
2. The Coryneform bacterium according to claim 1 , which has been modified so that the dehydroshikimate dehydratase activity is reduced.
3. The Coryneform bacterium according to claim 2 , wherein said gene encoding dehydroshikimate dehydratase is a DNA comprising the nucleotide sequence shown in SEQ ID NO: 15 or a DNA which hybridizes with a complementary sequence of said nucleotide sequence shown in SEQ ID NO: 15 under stringent conditions comprising hybridization under a salt concentration equivalent to 1×SSC and 0.1% SDS at 60° C. and encodes a protein having dehydroshikimate dehydratase activity.
4. The Coryneform bacterium according to claim 1 , wherein said aromatic carboxylic acid is a hydroxybenzene carboxylic acid.
5. The Coryneform bacterium according to claim 4 , wherein said aromatic carboxylic acid is protocatechuic acid.
6. The Coryneform bacterium according to claim 1 , wherein the at least one enzyme activity is reduced by disrupting a gene encoding the at least one enzyme.
7. The Coryneform bacterium according to claim 1 , wherein the at least one enzyme activity is reduced by mutating a gene encoding the at least one enzyme.
8. The Coryneform bacterium according to claim 1 , wherein the at least one enzyme activity is reduced by modifying an expression control sequence of a gene encoding the at least one enzyme.
9. A Coryneform bacterium, which has an ability to produce an organic acid and has been modified so that dehydroquinate synthase activity is reduced as compared to an unmodified strain, and the production of an aromatic carboxylic acid is thereby reduced, wherein the dehydroquinate synthase activity is reduced by disrupting a gene encoding the dehydroquinate synthase, mutating a gene encoding the dehydroquinate synthase, or modifying an expression control sequence of a gene encoding the dehydroquinate synthase.
10. The Coryneform bacterium according to claim 9 , which has further been modified so that at least one additional enzyme activity selected from the group consisting of dehydroquinate dehydratase activity and dehydroshikimate dehydratase activity is reduced as compared to an unmodified strain, and the production of an aromatic carboxylic acid is thereby reduced, wherein the at least one enzyme activity is reduced by disrupting a gene encoding the at least one enzyme, mutating a gene encoding the at least one enzyme, or modifying an expression control sequence of a gene encoding the at least one enzyme.
11. The Coryneform bacterium according to claim 1 , wherein the fermentable sugar is glucose, fructose, or sucrose.
12. The Coryneform bacterium according to claim 1 , wherein the fermentable sugar is glucose, or sucrose.
13. A method of producing a polymer of an organic acid, which comprises performing a polymerization reaction with an organic acid obtained by allowing the Coryneform bacterium according to claim 1 , an immobilized bacterium of said bacterium, a homogenate of the bacterium, or a fraction of the bacterium to act on an organic raw material.
14. The method according to claim 1 , further comprising crystallizing the organic acid.
15. The method according to claim 1 , wherein said aromatic carboxylic acid is a hydroxybenzene carboxylic acid.
16. The method according to claim 1 , wherein said organic acid is succinic acid.
17. The method according to claim 16 , wherein said polymer is a polyester or a polyamide.
18. A method of producing an organic acid, comprising allowing the Coryneform bacterium according to claim 1 , an immobilized bacterium of said bacterium, a homogenate of the bacterium, or a fraction of the bacterium to act on the fermentable sugar.
19. The method according to claim 18 , which is performed in an anaerobic atmosphere.
20. The method according to claim 18 , wherein said organic acid is succinic acid.
21. The method according to claim 18 , further comprising crystallizing said organic acid.
22. The method according to claim 18 , wherein said aromatic carboxylic acid is a hydroxybenzene carboxylic acid.