Monoclonal antibody capable of binding integrin alpha 10 beta 1
The present invention provides a monoclonal antibody or a fragment thereof binding to the extracellular I-domain of integrin alpha10beta1 and a hybridoma cell line deposited at the Deutsche Sammlung von Microorganismen and Zellkulturen GmbH under the accession number DSM ACC2583. Furthermore, the present invention also provides a monoclonal antibody or a fragment thereof binding to the extracellular I-domain of integrin alpha10beta1 produced by the hybridoma cell line deposited. Methods and uses of said antibody or a fragment thereof in identifying and selecting cells of a chondrogenic nature for treatment purposes, in particular for the identification and isolation of chondrocytes, mesenchymal progenitor cells and embryonic stem cells for tissue engineering of cartilage, or for identifying diagnostic and therapeutic tools in studying the biological role and the structural/functional relationships of the integrin alpha10beta1 with its various extracellular matrix ligands are also included.
1. A method for stimulating of the expression of collagen type II mRNA in a mammalian cell expressing integrin alpha10beta1, the method comprising the steps of:
a) providing a mammalian cell expressing integrin alpha10beta1,
b) contacting the cell with a monoclonal antibody or a fragment thereof under conditions wherein said monoclonal antibody or fragment thereof forms an antibody-antigen complex with an extracellular domain of integrin alpha10beta1 on said cell, wherein said antibody or fragment thereof binds specifically to an extracellular I-domain of an integrin alpha10 chain, and
c) incubating said antibody-antigen complex, thereby stimulating the expression of collagen type II mRNA.
2. The method of claim 1 , wherein said cell is a mesenchymal stem cell.
3. The method of claim 1 , wherein said cell is an embryonic stem (ES) cell.
4. The method of claim 1 , wherein said cell is a chondrocyte.
5. The method of claim 1 , wherein said cell is a fibroblast.
6. The method of claim 1 , wherein said monoclonal antibody or fragment thereof is the antibody produced by the hybridoma cell line deposited at the Deutsche Sammlung von Microorganismen and Zellkulturen GmbH under the accession number DSM ACC2583 or a fragment of the antibody produced by said hybridoma cell line.