Muscodor Albus Strain Producing Volatile Organic Compounds and Methods of Use
Disclosed herein is an isolated Muscodor albus strain producing volatile organic compounds such as aristolene, 3-octanone and/or acetic acid ester, as well as cultures of said strain and compositions, metabolites and volatiles derived from said strain or culture as well as methods of obtaining said compositions, metabolites and volatiles and their methods of use for controlling pests. Also disclosed are artificial compositions having the same components and uses as the volatiles derived from the strain. A method for capturing and sampling the volatiles is also disclosed.
1 . A liquid composition comprising at least one volatile organic compound produced by a Muscodor strain which:
(a) is capable of producing an acetic acid ester; and
(b) produces a product that possesses fungicidal, bacterial, nematicidal, and/or insecticidal activity.
2 . The liquid composition of claim 1 , wherein the at least one volatile organic compound is selected from:
acetic acid, 2-methylpropyl ester;
1-Butanol, 2-methyl-, acetate; and
acetic acid, 2-phenylethyl ester.
3 . The liquid composition of claim 1 , wherein the at least one volatile compound comprises
Ethanol
Ethyl acetate
1-propanol, 2-methyl
Butanal, 2-methyl
Propanoic acid, 2-methyl-, methyl ester
1-Butanol, 3-methyl-
1-Butanol, 2-methyl-
Acetic acid, 2-methylpropyl ester
1-Butanol, 3-methyl-, acetate
1-Butanol, 2-methyl-, acetate
4-Nonanone
2-Nonanone
Phenylethyl alcohol
Acetic acid, 2-phenylethyl ester
Cyclopeptane, 4-methylene-1-methyl-2-[2-methyl-1-propene-1-y]-1-vinyl
Azulene, 1,2,3,5,6,7,8,8a-octahydro-1,4-dimethyl-7-(1-methylethenyl)-,[1S-(1.alpha., 7.alpha., 8a.beta.)[-; and
1H-2-Benzopyran-1-one, 3,4-dihydro-8-hydroxy-3-methyl-, [R]-.
4 . The liquid composition of claim 1 , wherein the Muscodor strain is Muscodor albus strain SA-13 (NRRL Accession No. B-50774).
5 . The liquid composition of claim 1 , wherein the liquid composition is a whole cell broth.
6 . The liquid composition of claim 1 , wherein cells of the Muscodor strain have been removed.
7 . The liquid composition of claim 1 , wherein the at least one volatile organic compound is a synthetic compound.
8 . The liquid composition of claim 1 , wherein the composition comprises an artificial mixture of the at least one volatile organic compound.
9 . A method of producing a liquid composition of claim 1 , comprising:
(a) growing the Muscodor strain in a liquid medium; and
(b) obtaining the liquid medium.
10 . The method of claim 9 , further comprising removing cells of the Muscodor strain from the liquid medium.
11 . A method for modulating pest infestation and/or phytopathogenic infection in a plant comprising applying to the plant and/or seeds thereof and/or substrate used for growing said plant an effective amount of a liquid composition of claim 1 to modulate pest infestation and/or phytopathogenic infection of the plant.
12 . A composition comprising at least a first substance and a second substance, wherein
(a) the first substance is selected from a substantially pure culture, whole cell broth, cell fraction, supernatant, metabolite, or volatile organic compound derived from a Muscodor strain; and
(b) the second substance is a Trichoderma sp.
13 . The composition of claim 12 , wherein the Muscodor strain is Muscodor albus strain SA-13 (NRRL Accession No. B-50774).
14 . A method for modulating pest infestation and/or phytopathogenic infection in a plant comprising applying to the plant and/or seeds thereof and/or substrate used for growing said plant an effective amount of at least a first substance and a second substance to modulate pest infestation and/or phytopathogenic infection of the plant, wherein (a) the first substance is selected from a substantially pure culture, whole cell broth, cell faction, supernatant, metabolite, or volatile organic compound derived from a Muscodor strain; and (b) the second substance is a Trichoderma sp.
15 . The method of claim 14 , wherein the Muscodor strain is Muscodor albus strain SA-13 (NRRL Accession No. B-50774).
16 . The method according to claim 11 , wherein said pest is an insect, fungus, bacterium, or nematode.
17 . The method according to claim 14 , wherein said pest is an insect, fungus, bacterium, or nematode.
18 . The composition of claim 1 , wherein the composition is a biofumigant.
19 . The composition of claim 12 , wherein the composition is a biofumigant.