Major QTLs conferring resistance of corn to fijivirus
The invention relates to methods and compositions for identifying maize plants that have newly conferred resistance or enhanced resistance to, or are susceptible to, a Fijivirus, particularly Mal de Río Cuarto Virus (MRCV) and/or Maize Rough Dwarf Virus (MRDV). The methods use molecular genetic markers to identify, select and/or construct resistant plants or identify and counter-select susceptible plants. Maize plants that display newly conferred resistance or enhanced resistance to a Fijivirus that are generated by the methods of the invention are also a feature of the invention.
1. A method of identifying a maize plant or germplasm that displays newly conferred resistance or enhanced resistance to a member of Serogroup 2 of Fijivirus, the method comprising:
a. Isolating nucleic acids from a maize plant or germplasm;
b. analyzing the isolated nucleic acids for the presence of a QTL allele associated with the newly conferred resistance or enhanced resistance to a member of Serogroup 2 of Fijivirus, wherein the presence of said QTL allele is determined by detecting in the maize plant or germplasm a haplotype within a chromosomal interval flanked by and including MZA8381 and MZA18180, wherein said haplotype comprises a “G” at MZA16656-19-A, a “G” at MZA15490-801-A, a “C” at MZA15490-137-A, an “A” at MZA2038-71-A, a “T” at MZA2038-76-A, an “A” at MZA11826-801-A, a “C” at MZA11826-803-A, and an “A” at MZA9105-8-A, and
c. selecting said maize plant or germplasm if said QTL allele is detected.
2. The method of claim 1 , wherein the haplotype lies within a chromosomal interval flanked by and including MZA4305 and MZA2803.
3. The method of claim 1 , further comprising introgressing the haplotype in said maize plant or germplasm into a second maize plant or germplasm to produce an introgressed maize plant or germplasm.
4. The method of claim 1 , wherein the member of Serogroup 2 of Fijivirus is Mal de Río Cuarto Virus (MRCV).
5. The method of claim 1 , wherein the member of Serogroup 2 of Fijivirus is Maize Rough Dwarf Virus (MRDV).