PLASMA PROTEIN CONCENTRATE FOR CELL DELIVERY IN REGENERATIVE APPLICATIONS
The invention is directed to concentrating autologously-derived plasma, using the concentrated plasma fluid to dilute the patient's cells and applying the combination of concentrated fluid with cells at a site of pathology or mixing the combination of concentrated fluid with cells with a particulate material like a bone void filler prior to placing the mixture at a site of pathology.
1 . A method of preparing an enriched plasma-derived fluid, the method comprising:
preparing plasma protein concentrate;
preparing regenerative cells; and
combining the plasma protein concentrate with the regenerative cells to form an enriched plasma-derived fluid.
2 . The method of claim 1 , wherein the plasma protein concentrate is prepared by filtration, ultracentrifugation, cold precipitation or lyophilization.
3 . The method of claim 1 , wherein the regenerative cells are derived from bone, bone marrow, adipose, dermis or any combination thereof.
4 . The method of claim 1 , wherein the plasma protein concentrate is derived from peripheral blood or bone marrow aspirate.
5 . A method for improving cell adhesion and retention on a substrate, the method comprising the steps of:
coating plasma protein concentrate on a surface of the substrate;
applying cells or protein on the coated substrate; and
assessing the adhesion and retention of the cells or protein on the coated substrate.
6 . The method of claim 5 , wherein the plasma protein concentrate is prepared by filtration, ultracentrifugation, cold precipitation or lyophilization.
7 . The method of claim 5 , wherein the regenerative cells are derived from bone, bone marrow, adipose, dermis or any combination thereof.
8 . The method of claim 5 , wherein the plasma protein concentrate is derived from peripheral blood or bone marrow aspirate.
9 . A method for improving cell adhesion and retention in a treatment site, the method comprising;
preparing an enriched plasma-derived fluid;
introducing the enriched plasma-derived fluid to a treatment site; and
applying regenerative cells or proteins to the treatment site.
10 . The method of claim 9 , wherein the plasma protein concentrate is prepared by filtration, ultracentrifugation, cold precipitation or lyophilization.
11 . The method of claim 9 , wherein the regenerative cells are derived from bone, bone marrow, adipose, dermis or any combination thereof.
12 . The method of claim 9 , wherein the plasma protein concentrate is derived from peripheral blood or bone marrow aspirate.
13 . A method for improving cell adhesion and retention in a treatment site, the method comprising;
preparing an enriched plasma-derived fluid;
preparing regenerative cells;
mixing the enriched plasma-derived fluid with the regenerative cells; and
introducing the mixture of the enriched plasma-derived fluid and regenerative cells to a treatment site.
14 . The method of claim 13 , wherein the plasma protein concentrate is prepared by filtration, ultracentrifugation, cold precipitation or lyophilization.
15 . The method of claim 13 , wherein the regenerative cells are derived from bone, bone marrow, adipose, dermis or any combination thereof.
16 . The method of claim 13 , wherein the plasma protein concentrate is derived from peripheral blood or bone marrow aspirate.
17 . A method for improving cell adhesion and retention in a treatment site, the method comprising;
preparing a particulate material;
preparing regenerative cells;
mixing the particulate material with the regenerative cells; and
introducing the mixture of the enriched plasma-derived fluid and regenerative cells to a treatment site.
18 . The method of claim 17 wherein the particulate material is bone void filler.