Immunoassays for meperidine and metabolites
View Patent ↗The invention provides novel haptens and immunogens for the preparation of novel monoclonal antibodies, which detect the synthetic opioid meperidine and its active metabolite normeperidine. These antibodies enable methods and kits, which are useful in an immunoassay for therapeutic drug monitoring (TDM) and in extending the window of detection for cases of abuse and drug-facilitated sexual assault (DFSA).
1. A competitive immunoassay method of detecting or determining meperidine and/or normeperidine in a sample, the method comprising:
contacting the sample with the monoclonal antibody produced by the hybridoma cell line NM4.3F5.C10.B7.B2.B2.C4 deposited with the European Collection of Cell Cultures (ECACC) under deposit reference number 12090501, and with a detecting agent, wherein the detecting agent is N-(carboxyethyl) normeperidine conjugated through its carboxyl group to horseradish peroxidase; and
determining the presence and/or amount of meperidine and/or normeperidine in the sample by determining an amount of the detecting agent bound to the monoclonal antibody.