Chemiluminescent compositions and enhancing reagents for chemiluminescence
View Patent ↗An enhancing reagent for enhancing chemiluminescence of 1,2-dioxetane compounds and a method for using the enhancing reagent to enhance the chemiluminescence are provided, in which the enhancing reagent contains an alkyl bis-quaternary ammonium salt of Formula I. A chemiluminescent composition with a 1,2-dioxetane compound as a substrate and a kit thereof are further provided, which contain a 1,2-dioxetane compound and an alkyl bis-quaternary ammonium salt of Formula I.
1. A chemiluminescent composition comprising a 1,2-dioxetane compound and at least one alkyl bis-quaternary ammonium salt having the structure of General Formula I:
wherein:
R 1 is selected from at least one of the following: C 10-20 alkyl, C 10-20 alkenyl, C 10-20 alkynyl, and C 3-8 cycloalkylC 6-12 alkyl;
each n is an integer independently selected from 2 to 15;
each m is an integer independently selected from 1 to 6;
R 2 , R 3 , R 4 , R 5 , R 6 , and R 7 are each independently selected from at least one of the following: C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, and C 3-8 cycloalkyl; and
X − is a negative ion.
2. The chemiluminescent composition of claim 1 , wherein R 1 is selected from C 12-18 alkyl.
3. The chemiluminescent composition of claim 1 , wherein R 2 , R 3 , R 4 , R 5 , R 6 , and R 7 are each independently selected from methyl or ethyl.
4. The chemiluminescent composition of claim 1 , wherein R 2 , R 3 , R 4 , R 5 , R 6 , and R 7 are alkyl, each having the same number of carbon atoms.
5. The chemiluminescent composition of claim 1 , wherein each n is an integer independently selected from 5 to 10, or each m is independently selected from 2 or 3.
6. The chemiluminescent composition of claim 1 , wherein X − is selected from at least one of the following: halide ions, ClO 4 − , PF 6 − , CF 3 SO 3 − , BF 4 − , acetate, and p-toluene sulfonate anions.
7. The chemiluminescent composition of claim 1 , wherein the alkyl bis-quaternary ammonium salt compound is selected from at least one of the following: dodecylamino polyoxyethylene ether ammonium dichloride, octadecylamino polyoxyethylene ether ammonium dichloride, dodecylamino polyoxyethylene ether ammonium dibromide, octadecylamino polyoxyethylene ether ammonium dibromide, and a combination thereof.
8. The chemiluminescent composition of claim 1 , wherein the concentration of the alkyl bis-quaternary ammonium salt is in a range of between about 0.1 g/L and about 10 g/L.
9. The chemiluminescent composition of claim 1 , wherein the concentration of the alkyl bis-quaternary ammonium salt is in a range of between about 1 g/L and about 10 g/L.
10. The chemiluminescent composition of claim 1 , further comprising at least one of the following: a fluorescent agent, a magnesium ion, a buffer, and a preservative.
11. The chemiluminescent composition of claim 10 , wherein the buffer is 2-amino-2-methyl-1-propanol.
12. A chemiluminescent enhancing reagent configured to enhance the chemiluminescence of a 1,2-dioxetane compound, comprising:
at least one alkyl bis-quaternary ammonium salt having the structure of General Formula I:
wherein:
R 1 is selected from at least one of the following: C 10-20 alkyl, C 10-20 alkenyl, C 10-20 alkynyl, and C 3-8 cycloalkylC 6-12 alkyl;
each n is an integer independently selected from 2 to 15;
each m is an integer independently selected from 1 to 6;
R 2 , R 3 , R 4 , R 5 , R 6 , and R 7 are each independently selected from at least one of the following: C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, and C 3-8 cycloalkyl; and
X − is a negative ion; and
a solvent configured to dissolve the alkyl bis-quaternary ammonium salt and the 1,2-dioxetane compound.
13. The chemiluminescent enhancing reagent of claim 12 , wherein the enhancing reagent further comprises at least one of the following: a fluorescent agent, a magnesium ion, a buffer, and a preservative.
14. A kit for enhancing the chemiluminescence of 1,2-dioxetane compounds, comprising: at least one alkyl bis-quaternary ammonium salt having the structure of General Formula I:
wherein:
R 1 is selected from at least one of the following: C 10-20 alkyl, C 10-20 alkenyl, C 10-20 alkynyl, and C 3-8 cycloalkylC 6-12 alkyl;
each n is an integer independently selected from 2 to 15;
each m is an integer independently selected from 1 to 6;
R 2 , R 3 , R 4 , R 5 , R 6 , and R 7 are each independently selected from at least one of the following: C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, and C 3-8 cycloalkyl; and
X − is a negative ion; and
a manual instructing the user to mix the alkyl bis-quaternary ammonium salt with the 1,2-dioxetane compound.
15. The kit of claim 14 , wherein the components of the kit are stored individually or in the form of one or more mixtures.