Soybean event DP-305423-1 and compositions and methods for the identification and/or detection thereof
Compositions and methods related to transgenic high oleic acid/ALS inhibitor-tolerant soybean plants are provided. Specifically, the present invention provides soybean plants having a DP-305423-1 event which imparts a high oleic acid phenotype and tolerance to at least one ALS-inhibiting herbicide. The soybean plant harboring the DP-305423-1 event comprises genomic/transgene junctions having at least the polynucleotide sequence of SEQ ID NO:8, 9, 14, 15, 20, 21, 83 or 84. The characterization of the genomic insertion site of the DP-305423-1 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the soybean DP-305423-1 events are provided.
1. A soybean plant or seed comprising a polynucleotide having 99% identity to nucleotides 2899-7909 of SEQ ID NO: 82 and further comprising SEQ ID NO: 83 and SEQ ID NO: 84.
2. The soybean plant or seed of claim 1 , wherein the seed has an oleic acid content greater than 70% and a progeny seed produced by the plant has an oleic acid content greater than 70%.
3. The soybean plant or seed of claim 1 , wherein the soybean plant or seed further comprises a polynucleotide having 99% identity to nucleotides 18,652-31,579 of SEQ ID NO: 5.
4. The soybean plant or seed of claim 3 , wherein the soybean plant or seed further comprises a polynucleotide having 99% identity to nucleotides 12,164-14,494 of SEQ ID NO:6.
5. The soybean plant or seed of claim 4 , wherein the soybean plant or seed further comprises a polynucleotide having 99% identity to nucleotides 5751-7813 of SEQ ID NO:7.
6. The soybean plant or seed of claim 5 , wherein the seed has an oleic acid content greater than 70% and a progeny seed produced by the plant has an oleic acid content greater than 70%.
7. The soybean plant or seed of claim 6 , wherein the plant is tolerant to an ALS-inhibitor herbicide and a plant grown from the seed is tolerant to an ALS-inhibitor herbicide.
8. The soybean plant or seed of claim 3 , wherein the plant is tolerant to an ALS-inhibitor herbicide and a plant grown from the seed is tolerant to an ALS-inhibitor herbicide.
9. The soybean plant or seed of claim 8 , wherein the seed has an oleic acid content greater than 70% and a progeny seed produced by the plant has an oleic acid content greater than 70%.
10. The soybean plant or seed of claim 3 , wherein the seed has an oleic acid content greater than 70% and a progeny seed produced by the plant has an oleic acid content greater than 70%.
11. The soybean plant or seed of claim 1 , wherein the seed has an oleic acid content of 68.7% to 79.4% and a progeny seed produced by the plant has an oleic acid content 68.7% to 79.4%.
12. The soybean plant or seed of claim 5 , wherein the seed has an oleic acid content of 68.7% to 79.4% and a progeny seed produced by the plant has an oleic acid content 68.7% to 79.4%.
13. A method of producing a plant comprising high oleic seed, the method comprising breeding the plant of claim 1 , and selecting progeny by analyzing for progeny that comprises the polynucleotide having 99% identity to nucleotides 2899-7909 of SEQ ID NO: 82, wherein the progeny comprises SEQ ID NO: 83 and SEQ ID NO: 84 and further comprises high oleic seed.
14. A soybean plant or seed comprising a polynucleotide having 99% identity to nucleotides 18,652-31,579 of SEQ ID NO: 5 and further comprising SEQ ID NO: 8 and SEQ ID NO: 9.
15. The soybean plant or seed of claim 14 , wherein the plant is tolerant to an ALS-inhibitor herbicide and a plant grown from the seed is tolerant to an ALS-inhibitor herbicide.
16. The soybean plant or seed of claim 14 , wherein the soybean plant or seed further comprises a polynucleotide having 99% identity to nucleotides 2899-7909 of SEQ ID NO: 82.
17. The soybean plant or seed of claim 16 , wherein the seed has an oleic acid content greater than 70% and a progeny seed produced by the plant has an oleic acid content greater than 70%.
18. A method for controlling weeds in an area of cultivation, the method comprising applying an effective amount of an ALS inhibitor to the area of cultivation comprising the soybean plant of claim 14 .
19. A method of producing an ALS inhibitor tolerant plant, the method comprising breeding the plant of claim 14 , and selecting progeny by analyzing for progeny that comprise a polynucleotide comprising nucleotides 18,652-31,579 of SEQ ID NO: 5 wherein the progeny comprises SEQ ID NO: 8 and SEQ ID NO: 9 and further comprises tolerance to an ALS inhibitor.
20. A method for controlling weeds in an area of cultivation, the method comprising applying an effective amount of at least one sulfonylurea herbicide and at least one imidazolinone herbicide to the area of cultivation comprising the soybean plant of claim 15 .