AXMI-205 pesticidal gene and method for its use
View Patent ↗Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 3, or 4, the nucleotide sequence set forth in SEQ ID NO:1, 9, 10, or 11, as well as variants and fragments thereof.
1. A method for isolating or recovering from a sample containing nucleic acid sequences a nucleotide sequence encoding a polypeptide with a pesticidal activity comprising:
(a) providing a polynucleotide probe comprising the complement of the nucleic acid sequence of any of SEQ ID NO: 1, 9, 10, 11 or 12, or a fragment thereof comprising at least 500 consecutive nucleotides of SEQ ID NO: 1, 9, 10, 11 or 12;
(b) contacting the sample with the polynucleotide probe under stringent hybridization conditions, wherein said conditions comprise hybridization for 4 to 12 hours in 50% formamide, 1 M NaCl, 1% SDS at 37° C., and a wash in 0.1×SSC at 60 to 65° C.;
(c) isolating or recovering from said sample a nucleic acid that specifically hybridizes with the polynucleotide probe of (a); and
(d) assaying the polypeptide encoded by the nucleic acid of step (c) for pesticidal activity.