Automated device for point-of-care cell processing
The invention is directed to a device for the concentration of fluids comprising a donor receptacle containing a fluid to be concentrated, the donor receptacle comprising a plunger; a receiving receptacle to receive the concentrated fluid, the receiving receptacle comprising a plunger; a concentrator device that is connected to the donor receptacle and the receiving receptacle; and a housing that encloses the donor and receiving receptacles and the concentrator device that the plungers of the donor and receiving receptacles are enclosed within chambers that are gas tight.
1. A device for concentrating fluids comprising:
a disposable syringe donor receptacle containing a fluid to be concentrated, the disposable syringe donor receptacle comprising a donor plunger, the donor plunger comprising a first plunger head sealingly engaged with a receptacle of the disposable syringe donor receptacle;
a disposable syringe receiving receptacle to receive the fluid to be concentrated from the disposable syringe donor receptacle, the disposable syringe receiving receptacle comprising a receiving plunger, the receiving plunger comprising a second plunger head sealingly engaged with a receptacle of the disposable syringe receiving receptacle;
a concentrator device that is connected to the disposable syringe donor receptacle and the disposable syringe receiving receptacle;
a housing comprising a first gas-tight chamber adapted to securely receive the donor plunger within the first gas-tight chamber and a second gas-tight chamber adapted to securely receive the receiving plunger within the second gas-tight chamber;
an electronic controller configured to control movement of the donor plunger and the receiving plunger by directing a pressurized fluid into the first and second gas-tight chambers; and
a valve coupled to the first and second gas-tight chambers to selectively direct the pressurized fluid to the donor plunger or the receiving plunger;
wherein each of the first and second gas-tight chambers comprises a configuration that allows the pressurized fluid to contact a back side of the first and second plunger head, respectively, to cause the first and second plunger head to move in response to the pressurized fluid contacting the back side of the first and second plunger head.
2. The device of claim 1 , wherein the concentrator device is a hollow fiber concentrator device.
3. The device of claim 1 , further comprising a dewatering syringe disposed within the housing and coupled to the concentrator device.
4. The device of claim 1 , further comprising a second dewatering syringe disposed within the housing and coupled to the concentrator device.
5. The device of claim 1 , further comprising:
a cycle counter coupled to the electronic controller; and
wherein the electronic controller records a number of concentration cycles and displays the number of concentration cycles on the cycle counter.
6. The device of claim 1 , wherein the electronic controller is programmed to perform a selected number of cycles.
7. The device of claim 1 , further comprising a first sensor array aligned along a wall of the first gas-tight chamber and aligned adjacent to the donor plunger and a second sensor array disposed on a wall of the second gas-tight chamber and aligned adjacent to receiving plunger, wherein the first sensor array monitors a distance the donor plunger travels to track a reduced displacement of the donor plunger and the second sensor array monitors a distance the receiving plunger travels to track a reduced displacement of the receiving plunger.
8. The device of claim 7 , wherein the first sensor array comprises:
a light source; and
a sensor positioned so that the donor plunger is between a light path of the light source and the sensor and capable of detecting light emitted from the light source such that, as the donor plunger moves between the light source and the sensor, the sensor detects movement of the donor plunger.