Methods and compositions for diagnosing complications of pregnancy
The present invention provides methods and compositions for identifying subjects at risk of developing a complication of pregnancy, such as preeclampsia or preterm labor. The compositions are microRNAs and associated nucleic acids.
1. A method for determining whether a pregnant female subject is at risk of developing preeclampsia, said method comprising: obtaining a test biological sample from said female subject and a control biological sample from a pregnant woman of same gestational age with no clinical or laboratory evidence of hypertensive disorder of pregnancy; extracting RNA from the test biological sample and the control biological sample; determining by real-time PCR an expression level of SEQ ID NO: 10 in the test biological sample, wherein said real-time PCR comprises contacting the test biological sample with forward and reverse primers, wherein the forward primer comprises 15-21 nucleotides identical to a DNA sequence equivalent to SEQ ID NO: 10; comparing the expression level of SEQ ID NO: 10 in the test biological sample and the control biological sample; and determining whether said pregnant female is at risk of developing preeclampsia based on increased expression of SEQ ID NO: 10 in the test biological sample relative to the control biological sample.
2. The method of claim 1 , wherein said biological sample is selected from the group consisting of bodily fluid and a tissue sample.
3. The method of claim 2 , wherein said bodily fluid sample is serum sample.
4. The method of claim 2 , wherein said tissue is a fresh, frozen, fixed, wax-embedded or formalin fixed paraffin-embedded (FFPE) tissue.
5. The method of claim 4 , wherein said tissue sample is placenta sample or uterine myometrium sample.