Apparatus and method for increasing collection efficiency in capillary based flowcytometry
View Patent ↗In a particle analyzing apparatus including a capillary for passing through a fluid containing particles to be analyzed, an optical system is employed to collect fluorescent light emitted from particles or substances labeled to the particles with improved collection efficiency preserving resolution of the instrument. The optical system may include a first collection lens attached to the capillary and a first reflection element arranged adjacent to the first collection lens configured to reflect fluorescent light of one or more wavelengths. The optical system may include a second collection lens attached to the capillary and a second reflection element arranged adjacent to the second collection lens configured to reflect fluorescent light of one or more wavelengths.
1. A flow cell for use in a particle analyzing apparatus, comprising:
a capillary having a wall extending in a longitudinal direction defining a bore configured to pass a fluid containing particles to be analyzed, said particles fluoresce or are labeled with substances that fluoresce upon illumination of light; and
a first collection lens attached to the wall of the capillary configured to collect fluorescent light emitted from a particle or substance labeled to the particle; and
a second collection lens attached to the wall of the capillary opposite to the first collection lens configured to collect fluorescent light emitted from particles or substances labeled to the particles.
2. The flow cell of claim 1 wherein the wall of the capillary has a first substantially flat surface, and the first collection lens has a planar surface which is attached to the first flat surface of the wall.
3. The flow cell of claim 1 wherein at least one of the first and second collection lenses is coupled to the capillary by optical contact.
4. The flow cell of claim 1 wherein at least one of the first and second collection lenses is coupled to the capillary with a substance which has a refractive index that substantially matches with a refractive index of the capillary.
5. The flow cell of claim 1 wherein the capillary has a cross section transverse to the longitudinal direction, the cross section having an outer shape of a square or rectangle.
6. The flow cell of claim 5 wherein the cross section has an inner shape of a circle or square.
7. The flow cell of claim 6 wherein the cross section has an outer shape of a rectangle and an inner shape of a circle.
8. The flow cell of claim 1 wherein the wall of the capillary comprises four substantially flat outer surfaces and a circular inner surface, said first collection lens is attached to a first flat surface, and the second collection lens attached to a second flat surface opposite to the first flat surface configured to collect fluorescent light emitted from particles or substances labeled to the particles.