Multi-chamber freezing bag
Methods for preparing and using multiple chambered freezing bags containing biological fluid in the chambers are disclosed.
1. A method for sealing a freezing bag having multiple compartments containing biological fluid, the method comprising:
providing a freezing bag comprising opposing side walls having exterior surfaces, a top, and a bottom, and multiple compartments, each compartment having an interior volume containing biological fluid therein, the bag further comprising a passageway providing fluid communication between the compartments,
inserting the freezing bag between first and second panels of an open jig, the first and second panels each having an outside surface and an inside surface, the inside surfaces forming an interior of the jig, the first and second panels each having a plurality of cut outs at one end of the panels;
closing the jig with the bag located in the interior of the jig, such that the inside surfaces of the first and second panels contact the exterior surfaces of the opposing side walls of the bag; and
applying heat to the bag through the plurality of cut outs;
wherein applying heat seals the passageway at multiple locations and forms a plurality of sealed fluidly isolated biological fluid containing compartments in the bag, each compartment containing about the same volume of biological fluid.
2. The method of claim 1 , wherein the panels are connected by a hinge, and closing the jig comprises pivoting one of the first or second panels toward the other of the first or second panels.
3. The method of claim 2 , comprising forming at least 3 sealed fluidly isolated biological fluid containing compartments in the bag, each compartment containing about the same volume of biological fluid.
4. The method of claim 3 , further comprising freezing the freezing bag comprising the plurality of sealed fluidly isolated biological fluid containing compartments.
5. The method of claim 2 , further comprising freezing the freezing bag comprising the plurality of sealed fluidly isolated biological fluid containing compartments.
6. The method of claim 1 , comprising forming at least 3 sealed fluidly isolated biological fluid containing compartments in the bag, each compartment containing about the same volume of biological fluid.
7. The method of claim 6 , further comprising freezing the freezing bag comprising the plurality of sealed fluidly isolated biological fluid containing compartments.
8. The method of claim 1 , further comprising freezing the freezing bag comprising the plurality of sealed fluidly isolated biological fluid containing compartments.