IP Library › Patent Application 14072491
Patent Application
App. No. 14/072,491

ENDOCRINE PRECURSOR CELLS, PANCREATIV HORMONE-EXPRESSING CELLS AND METHOS OF PRODUCTION

Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US None
App. No.
14/072,491
Abstract

Disclosed herein are cell cultures and enriched cell populations of endocrine precursor cells, immature pancreatic hormone-expressing cells and mature pancreatic hormone-expressing cells. Also disclosed herein are methods of producing such cell cultures and cell populations.

Claims (21)

1 - 20 . (canceled)

21 . A population of cells expressing markers characteristic of the pancreatic endoderm lineage, wherein said markers are pancreatic duodenal homeobox 1(PDX1) and NKX6 transcription factor related locus 1 (NKX6.1) or pancreas specific transcription factor 1a(PTF1A).

22 . The cell population of claim 1 , wherein said population is derived from a human pluripotent stem cell.

23 . The cell population of claim 1 , wherein said population is in contact with a gamma secretase inhibitor.

24 . The cell population of claim 1 , wherein said population is in contact with a retinoid.

25 . The cell population of claim 1 , wherein said population is in contact with noggin.

26 . The cell population of claim 1 , wherein said markers are PDX1 and NKX6.1.

27 . The cell population of claim 1 , wherein said markers are PDX1, NKX6.1 and PTF1A.

28 . The cell population of claim 1 , wherein the population does not substantially express INS or GCG.

29 . An in vitro cell culture comprising pancreatic endoderm cells that express a marker selected from the group consisting of PDX1, NKX6.1 and PTF1A.

30 . The cell culture of claim 29 , wherein said cells are in contact with a retinoid.

31 . The cell culture of claim 29 , wherein said cells are in contact with noggin.

32 . The cell culture of claim 29 , wherein said pancreatic endoderm cells express PDX1 and NKX6.1.

33 . The cell culture of claim 29 , wherein said cells are in contact with a gamma secretase inhibitor.

34 . The cell culture of claim 29 , wherein the pancreatic endoderm cells do not substantially express INS or GCG.

35 . An in vitro differentiated population of pancreatic endoderm cells obtained from the differentiation of pluripotent stem cells, wherein the population expresses PDX1 and NKX6.1 or PTF1A.

36 . The cell population of claim 35 , wherein said pluripotent stem cell is an embryonic stem cell.

37 . The cell population of claim 35 , wherein said population is in contact with a gamma secretase inhibitor.

38 . The cell population of claim 35 , wherein said population is in contact with a retinoid.

39 . The cell population of claim 35 , wherein said population is in contact with noggin.

40 . The cell population of claim 35 , wherein said population does not substantially express INS or GCG.