Metastasis specific splice variants of Mena and uses thereof in diagnosis, prognosis and treatment of tumors
View Patent ↗Methods and kits for diagnosis, prognosis and treatment of metastatic tumors are provided where the metastatic tumor is characterized by changes in expression of +++, ++ and/or 11a variants of Mena.
1. A kit comprising a nucleic acid encoding the +++ isoform of Mena, wherein the nucleic acid consists of SEQ ID NO: 3, or the entire complement thereof, wherein the nucleic acid is labeled with a fluorescent label or a radiolabel attached to the nucleic acid.
2. The kit of claim 1 , further comprising a probe or PCR primers that specifically hybridize to nucleic acid encoding Mena 11a, wherein Mena 11a consists of SEQID NO: 24.
3. The kit of claim 2 , wherein the nucleic acid encoding Mena 11a consists of SEQ ID NO:25.
4. The kit of claim 1 , further comprising a probe or PCR primers that specifically hybridize to nucleic acid encoding pan Mena.
5. The kit of claim 1 , further comprising a forward primer for the ++ isoform of Mena that consists of SEQ ID NO: 7 and a reverse primer for the ++ isoform of Mena that consists of SEQ ID NO: 8.
6. The kit of claim 1 , further comprising a forward primer for Mena 11a that consists of SEQ ID NO: 12 or 13, and a reverse primer for Mena 11a that consists of SEQ ID NO: 23.
7. The kit of claim 1 , further comprising a forward primer for pan Mena that consists of SEQ ID NO: 16 and a reverse primer for pan Mena consists of SEQ ID NO: 17.
8. The kit of claim 1 , further comprising a probe or PCR primers that specifically hybridize to nucleic acid encoding the ++ isoform of Mena, wherein the ++ isoform of Mena consists of SEQ ID NO: 2.
9. The kit of claim 8 , wherein the nucleic acid encoding the ++ isoform of Mena consists of SEQ ID NO: 1.
10. A kit comprising a pair of primers wherein the forward primer for the +++ isoform of Mena consists of SEQ ID NO: 7 and the reverse primer consists of SEQ ID NO: 10, wherein the nucleic acid is labeled with a fluorescent label or a radiolabel attached to the nucleic acid.
11. The kit of claim 10 , further comprising a probe or PCR primers that specifically hybridize to nucleic acid encoding Mena 11a, wherein Mena 11a consists of SEQ ID NO: 24.
12. The kit of claim 11 , wherein the nucleic acid encoding Mena 11a consists of SEQ ID NO:25.
13. The kit of claim 10 , further comprising a probe or PCR primers that specifically hybridize to nucleic acid encoding pan Mena.
14. The kit of claim 10 , further comprising a forward primer for the ++ isoform of Mena that consists of SEQ ID NO: 7 and a reverse primer for the ++ isoform of Mena that consists of SEQ ID NO: 8.
15. The kit of claim 10 , further comprising a forward primer for Mena 11a that consists of SEQ ID NO: 12 or 13, and a reverse primer for Mena 11a that consists of SEQ ID NO: 23.
16. The kit of claim 10 , further comprising a forward primer for pan Mena that consists of SEQ ID NO: 16 and a reverse primer for pan Mena consists of SEQ ID NO: 17.
17. The kit of claim 10 , further comprising a probe or PCR primers that specifically hybridize to nucleic acid encoding the ++ isoform of Mena, wherein the ++ isoform of Mena consists of SEQ ID NO: 2.
18. The kit of claim 17 , wherein the nucleic acid encoding the ++ isoform of Mena consists of SEQ ID NO: 1.