Temporal seed promoters for expressing genes in plants
View Patent ↗The present invention relates to the field of plant genetic engineering. More specifically, the present invention relates to seed specific gene expression during a defined period of embryogenesis. The present invention provides promoters capable of transcribing heterologous nucleic acid sequences in seeds, and methods of modifying, producing, and using the same.
1. A nucleic acid construct comprising a promoter operably linked to a second nucleic acid, wherein the promoter and second nucleic acid are heterologous with respect to each other, and wherein the promoter comprises SEQ ID NO:1 or the complement thereof.
2. A plant comprising the nucleic acid construct of claim 1 .
3. The plant of claim 2 , wherein said second nucleic acid is a structural nucleic acid.
4. The plant of claim 3 , wherein said structural nucleic acid encodes a protein selected from the group consisting of a seed storage protein, a fatty acid pathway enzyme, a tocopherol biosynthetic enzyme, an amino acid biosynthetic enzyme, a steroid pathway enzyme, and a starch branching enzyme.
5. The plant of claim 3 , wherein said structural nucleic acid encodes a protein selected from the group consisting of anthranilate synthase, tryptophan decarboxylase, threonine deaminase, and aspartate kinase.
6. The plant of claim 3 , wherein said structural nucleic acid encodes a starch branching enzyme.
7. The plant of claim 3 , wherein said structural nucleic acid is oriented to express an antisense RNA molecule.
8. The plant of claim 2 , wherein said plant is selected from the group consisting of canola, crambe , mustard, castor bean, sesame, cottonseed, linseed, maize, soybean, Arabidopsis phaseolus , peanut, alfalfa, wheat, rice, oat, sorghum , rapeseed, rye, tritordeum, millet, fescue, perennial ryegrass, sugarcane, cranberry, papaya , banana, safflower, oil palms, flax, muskmelon, apple, cucumber, dendrobium, gladiolus, chrysanthemum , liliacea, cotton, eucalyptus , sunflower, Brassica campestris, Brassica napus , turfgrass, sugarbeet, coffee, and dioscorea.
9. The plant of claim 2 , wherein said plant is soybean.
10. The plant of claim 3 , wherein said structural nucleic acid is expressed in an organ specific manner.
11. The plant of claim 10 , wherein said structural nucleic acid is expressed in a seed.
12. A method of producing a transformed plant comprising:
(a) providing the nucleic acid construct of claim 1 , wherein said second nucleic acid is a structural nucleic acid; and
(b) transforming a plant with said nucleic acid construct.
13. The method of claim 12 , wherein said plant produces a seed and said structural nucleic acid is transcribed in said seed.
14. A meal comprising the plant of claim 11 .
15. A feedstock comprising the plant of claim 11 .
16. An oil from the plant of claim 11 .
17. A cell containing the nucleic acid construct of claim 1 .
18. The cell according to claim 17 , wherein said cell is selected from the group consisting of a bacterial cell, a mammalian cell, an insect cell, a plant cell, and a fungal cell.
19. The cell according to claim 18 , wherein said cell is Agrobacterium tumefaciens.
20. A seed generated by a plant containing the nucleic acid construct of claim 1 , wherein the seed comprises the nucleic acid construct.