Polymer-based sustained release device
View Patent ↗This invention relates to compositions for the sustained release of biologically active polypeptides, and methods of forming and using said compositions, for the sustained release of biologically active polypeptides. The sustained release compositions of this invention comprise a biocompatible polymer having dispersed therein, a biologically active polypeptide and a sugar.
1. A method of preparing a composition for the sustained release of a glucoregulatory peptide comprising the steps of:
a) forming a mixture by combining:
an aqueous phase comprising a sugar and a water-soluble glucoregulatory peptide, with
an oil phase comprising a biocompatible polymer and a solvent for the biocompatible polymer;
b) forming a water-in-oil emulsion of the mixture from step a), wherein the inner emulsion droplet size is less than about 0.5 microns;
c) adding a coacervation agent to the mixture to form embryonic microparticles, wherein the coacervation agent is silicone oil added in an amount sufficient to achieve a silicone oil to polymer solvent ratio of from about 0.75:1 to about 2:1;
d) transferring the embryonic microparticles to a quench solvent to harden the microparticles;
e) collecting the hardened microparticles; and
f) drying the hardened microparticles,
wherein the glucoregulatory peptide is present in the composition at a concentration of about 0.01% to about 10% w/w based on the total weight of the composition, and the sugar is present in the composition at a concentration of about 0.01% to about 5% w/w based on the total weight of the composition.
2. The method of claim 1 , wherein water-in-oil emulsion of step b) is formed by homogenization.
3. The method of claim 1 , wherein the inner emulsion droplet size is less than or equal to about 0.4 microns.
4. The method of claim 3 , wherein the inner emulsion droplet size is from about 0.2 to about 0.4 microns.
5. The method of claim 1 , wherein the glucoregulatory peptide is selected from GLP-1, GLP-2, exendin-3, exendin-4, and combinations thereof.
6. The method of claim 1 , wherein the sugar is selected from sucrose, mannitol, and combinations thereof.
7. The method of claim 1 , wherein the biocompatible polymer is selected from the group consisting of poly(lactides), poly(glycolides), poly(lactide-co-glycolides), poly(lactic acid)s, poly(glycolic acid)s, poly(lactic acid-co-glycolic acid)s and blends and copolymers thereof.
8. The method of claim 1 , wherein the biocompatible polymer is present in the oil phase at about 10% w/v or less.
9. The method of claim 1 , wherein in step c), the silicone oil is added in an amount sufficient to achieve a silicone oil to polymer solvent ratio of from about 1:1 to about 1.5:1.
10. The method of claim 9 , wherein the ratio of silicone oil to polymer solvent is about 0.5:1.
11. The method of claim 1 , wherein the solvent biocompatible polymer is methylene chloride.
12. The method of claim 1 , wherein the quench solvent is a heptane/ethanol solvent mixture.