Passive microfluidic metering device
An integrated fluidic device comprising includes an input chamber that provides an input of a sample fluid, and a first overspill chamber in fluid communication with the input chamber. A metering conduit is in fluid communication with the fluid input chamber and the first overspill chamber. The metering conduit meters a first metered volume of fluid from the sample fluid, and the first overspill chamber receives fluid in excess of the first metered volume of fluid. A second overspill chamber is in fluid communication with the metering conduit. The metering conduit meters a second metered volume of fluid from the first metered volume of fluid, and the second overspill chamber receives fluid from the first metered volume of fluid in excess of the second metered volume of fluid. The second overspill chamber has a fluid actuated closable valve for controlling the metering of the second metered volume of fluid.
1. An integrated fluidic device comprising:
a sample fluid input chamber configured to provide an input of a sample fluid;
a first overspill chamber in fluid communication with the fluid input chamber;
a metering conduit in fluid communication with the fluid input chamber and the first overspill chamber, wherein the metering conduit is configured to meter a first metered volume of fluid from the sample fluid, and the first overspill chamber is configured to receive fluid from the sample fluid in excess of the first metered volume of fluid; and
a second overspill chamber in fluid communication with the metering conduit, wherein the metering conduit is configured to meter a second metered volume of fluid from the first metered volume of fluid, and the second overspill chamber is configured to receive fluid from the first metered volume of fluid in excess of the second metered volume of fluid;
wherein the second overspill chamber has a fluid actuated closable valve configured to isolate the second metered volume of fluid out of the first metered volume of fluid when the fluid actuated closable valve closes; and
wherein the first overspill chamber includes a first absorbent pad configured to absorb the fluid in excess of the first metered volume of fluid, and the fluid actuated closeable valve includes a second absorbent pad configured to absorb the fluid in excess of the second metered volume of fluid.
2. The integrated fluidic device of claim 1 , wherein the first overspill chamber is in fluid communication with the metering conduit via a first overspill conduit, and the first overspill conduit is offset from the metering conduit.
3. The integrated fluidic device of claim 1 , further comprising a blind fluidic conduit configured to split the second metered volume of fluid from the first metered volume of fluid.
4. The integrated fluidic device of claim 1 , further comprising an optical sensor device that measures a length of the second metered volume of fluid to validate the volume of the second metered volume of fluid.
5. The integrated fluidic device of claim 1 , further comprising:
a capillary stop at an end of the metering conduit opposite the fluid input chamber; and
a mixing chamber in fluid communication with the capillary stop, wherein the mixing chamber is configured to receive the second metered volume of fluid from the metering conduit through the capillary stop.
6. The integrated fluidic device of claim 5 , wherein the mixing chamber has a magnetic flea, and an aspect ratio of a length of the magnetic flea to a diameter of the mixing chamber is at least 0.5.
7. The integrated fluidic device of claim 5 , further comprising a closeable lid, and the first overspill chamber includes a vent hole, wherein the closeable lid is configured to close over the vent hole to seal the sample fluid input chamber and force at least a portion of the second metered volume of fluid into the mixing chamber.
8. The integrated fluidic device of claim 5 , further comprising at least one further fluid input chamber in fluid communication with the metering conduit, wherein the mixing chamber is configured to receive a further fluid from the at least one further fluid input chamber through the metering conduit and the capillary stop.
9. The integrated fluidic device of claim 8 , wherein the at least one further fluid chamber includes a pressure displacement mechanism configured to transfer the further fluid from the at least one further fluid chamber into the metering conduit.
10. The integrated fluidic device of claim 9 , wherein the at least one further fluid chamber has first and second frangible seals, and the pressure displacement mechanism is a moveable piston; and
wherein the moveable piston has a needle that is configured to pierce the first and second frangible seals to transfer the further fluid from the at least one further fluid chamber into the metering conduit.
11. The integrated fluidic device of claim 8 , wherein the integrated fluidic device has n further fluid input chambers, where n is greater than or equal to two.
12. The integrated fluidic device of claim 1 , further comprising:
an output conduit in fluid communication with the mixing chamber; and
a cell counter in fluid communication with the output conduit;
wherein the output conduit is configured to transfer fluid to the cell counter from the mixing chamber, and the cell counter is configured to count cells that are present in the fluid transferred from the mixing chamber.
13. The integrated fluidic device of claim 12 , further wherein the cell counter comprises at least one of a white blood cell counter or a hemoglobin measurement chamber.