Cell seeding device and method

1. A device comprising: an anchor comprising at least one sidewall comprising an upper portion that is disposed in a plane approximately parallel to the longitudinal axis (L) of the device and a lower portion with an outer surface of the lower portion angled radially toward the longitudinal axis (L) of the device wherein when a membrane is present, the lower portion engages at least a portion of the membrane: and at least one tab disposed on the upper portion of at least one sidewall, wherein the at least one tab is disposed in the same plane as the upper portion on which it is disposed; a tray including a well adapted to receive the anchor, wherein the anchor is adapted to be slidably disposed in the well; and a cover adapted to engage the tray and cover the well.

2. The device according to claim 1 , wherein the tray includes a top surface surrounding an opening of the well.

3. The device according to claim 2 , wherein the tray includes a flange disposed at least partially around a periphery of the top surface.

4. The device according to claim 3 , wherein the flange includes at least one first thread.

5. The device according to claim 4 , wherein the top surface includes a first rim formed at least partially around the periphery of the top surface.

6. The device according to claim 5 , wherein the cover includes a cavity adapted to receive the flange.

7. The device according to claim 6 , wherein the cavity includes at least one second thread adapted to engage the at least one first thread.

8. The device according to claim 6 , wherein the cavity includes a slot adapted to receive the first rim.

9. The device of claim 1 , wherein the well includes at least one first sidewall and a base.

10. The device according to claim 9 , wherein the base includes a second rim formed at least partially around a periphery of an external surface.

11. The device according to claim 9 , wherein a first cross-sectional area of the base is approximately equal to a second cross-sectional area of the space.

12. The device according to claim 1 , wherein the anchor includes a second sidewall enclosing a space.

13. The device according to claim 12 , wherein the cover includes at least one rib formed on an outer surface.

14. The device according to claim 1 , wherein the well is at least partially transparent and the anchor is at least partially colored.

15. The device of claim 1 , further comprising a membrane, wherein the membrane is in the well or adjacent to a base of the well.

16. The device of claim 15 , wherein the membrane comprises a substantially uniform distribution of cells.

17. The device of claim 1 , further comprising a membrane, wherein the anchor is on top of the membrane.

18. The device of claim 17 , wherein the membrane comprises a substantially uniform distribution of cells.

19. The device of claim 1 , further comprising a membrane, wherein the membrane is in the well or adjacent to a base of the well and wherein the anchor is on top of the membrane such that a mixture comprising cells is confined within a volume bounded by the sidewalls of the anchor and the membrane.

20. The device of claim 19 , wherein confining the mixture comprising cells promotes uniform cell distribution over the membrane.

21. A method of generating a membrane-cell matrix having a substantially uniform distribution of the cells on the membrane in at least two dimensions comprising:

providing the device of claim 1 ;

placing a membrane in the well;

placing the anchor in the well at least partially in contact with the membrane;

and filling the well at least partially with a medium containing cells.

22. The method according to claim 21 , further comprising: removing the anchor from the well; and removing the membrane-cell matrix.