Process for the isolation of rhamnolipids
A process for isolating rhamnolipids is provided. The process includes providing an aqueous medium containing at least one rhamnolipid and having a pH of less than 6. Next, the aqueous medium is brought into contact with at least one organic solvent to provide a multiphase system and then the aqueous phase is separated off. The pH is then increased to a value of 6 or more to provide a multiphase organic system. Next, a rhamnolipid-enriched organic phase is separated off. An optional step of further purifying the rhamnolipid may be performed.
1. A process for the isolation of rhamnolipids, said process comprising:
A) providing an aqueous medium containing at least one rhamnolipid and having a pH of less than 6, wherein the aqueous medium comprises between 10 g/l and 300 g/l of the at least one rhamnolipid,
B) i) bringing the aqueous medium into contact with at least one organic solvent to provide a multiphase system X, wherein the at least one organic solvent dissolved in the aqueous medium is in an amount from 0.1 to 30% by weight of the multiphase system X, ii) separating off the aqueous phase from multiphase system X, to provide an organic phase Y,
C) increasing the pH of the organic phase Y, to a value of 6 or more to provide a multiphase system Z comprising at least two organic phases, and
D) separating off a rhamnolipid-enriched organic phase from the multiphase system Z.
2. The process according to claim 1 , wherein said pH of said aqueous medium in process step A) is from 2 to 4.5.
3. The process according to claim 1 , wherein the at least one organic solvent of process step B) is selected from methyl acetate, ethyl acetate, propyl acetate, isopropyl acetate, butyl acetate, isoamyl acetate, butan-1-ol, butan-2-ol and diethyl ether.
4. The process according to claim 1 , wherein a volume ratio of the aqueous medium to the at least one organic solvent prior to bringing them into contact is from 0.2:1 to 5:1.
5. The process according to claim 1 , wherein in process step C) the pH is increased to 6.1 to 9.
6. The process according to claim 1 , wherein in process step C) the pH is increased by means of an inorganic base.
7. The process according to claim 1 , wherein process steps B), C) and D) are carried out in a temperature range from 10 to 50° C.
8. The process according to claim 1 , further comprising:
E) further purify the rhamnolipid within the rhamnolipid-rich phase.
9. The process according to claim 8 , wherein an organic solvent is used in process step E) to separated off from the rhamnolipid by means of at least partial evaporation.